《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (03): 233-235.

• 基础研究 • 上一篇    下一篇

脑外伤对骨折愈合中血管内皮生长因子表达的影响

刘志奎;张柳;姜晓华;穆树林;孙晓新   

  1. 华北煤炭医学院附属医院骨外科,河北唐山市 063000
  • 收稿日期:2008-11-06 出版日期:2009-03-01 发布日期:2009-03-01
  • 通讯作者: 张柳

Effect of Traumatic Brain Injury on Expression of Vascular Endothelial Growth Factor in Femoral Fracture Callus in Rats

LIU Zhi-kui, ZHANG Liu, JIANG Xiao-hua, et al   

  1. Department of Orthopedics, Hospital Affiliated to North China Coal Medical University, Tangshan 063000, Hebei, China
  • Received:2008-11-06 Published:2009-03-01 Online:2009-03-01

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摘要： 目的 探讨脑外伤对骨折愈合的影响及作用机制。方法 12周龄雌性SD大鼠随机分成2组：骨折合并脑外伤组和单纯骨折组。建立骨折及脑外伤模型，术后1周、2周、3周、4周截取骨痂，HE染色观察骨痂生长情况，免疫组织化学染色及原位杂交检测血管内皮生长因子（VEGF）及其mRNA表达。结果 HE染色示单纯骨折组呈典型骨折愈合过程，而骨折合并脑外伤组骨痂形成及改造提前。骨折合并脑外伤组术后1~4周骨痂中VEGF及其mRNA阳性细胞百分率分别高于同一时间点单纯骨折组（P<0.05）。结论 脑外伤对骨折愈合有促进作用，可能与脑外伤后VEGF表达水平升高有关。

关键词: 骨折, 愈合, 脑外伤, 血管内皮生长因子, 大鼠

Abstract: Objective To explore the effect of the traumatic brain injury on the fracture healing and the related mechanism. Methods 64 12-week-old SD rats were randomly divided into 2 groups: The femoral fracture with a brain injury group and the femoral fracture group. The bone callus was obtained 1, 2, 3 and 4 week after operation respectively, and observed with the HE staining. The vascular endothelial growth factor (VEGF) and VEGF mRNA in the callus were detected with immunohistochemical staining and hybridization in situ. Results The formation and reforming of the callus in group with brain injury were ahead to only fracture. The percentages of the cells positive for VEGF and VEGF mRNA in the callus were more in group with brain injury than in pure fracture group at the same time point (P<0.05). Conclusion Traumatic brain injury can promote the healing of fracture, which is probably related to an increase in the expression of VEGF.

Key words: fracture, healing, traumatic brain injury, vascular endothelial growth factor (VEGF), rats