《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (03): 228-230.

• 基础研究 • 上一篇    下一篇

正常与损伤条件下乳鼠原代心肌细胞培养上清液对人骨髓间充质干细胞向心肌样细胞的诱导作用

邓方阁1,2;郭连峰3;马英智1;李玉林1   

  1. 1.吉林大学白求恩医学院病理生物学教育部国家重点实验室，吉林长春市 130021;2.广州医学院第一附属医院广州呼吸疾病研究所呼吸疾病国家重点实验室，广东广州市 510120;3.奥林巴斯销售服务有限公司生命科学事业部，北京市 100022
  • 收稿日期:2008-10-17 出版日期:2009-03-01 发布日期:2009-03-01
  • 通讯作者: 李玉林

Inducing Human Bone Marrow Mesenchymal Stem Cells to Cardiomyo-Like Cells with Supernatant of Normal or Injured Rat Cardiomyocytes in Vitro

DENG Fang-ge, GUO Lian-feng, MA Ying-zhi, et al   

  1. Key Laboratory of Pathobiology, Jilin University, Changchun 130021,China
  • Received:2008-10-17 Published:2009-03-01 Online:2009-03-01

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摘要： 目的 体外观察正常及损伤状态下的心肌细胞（CMs）培养上清液诱导人骨髓间充质干细胞（hMSCs）向心肌样细胞分化的作用。方法 体外分离培养hMSCs及乳鼠原代CMs，制备正常搏动状态及损伤状态下的CMs培养上清液，分别对传代hMSCs培养诱导27~30 d。倒置显微镜观察两组的细胞形态，免疫细胞化学染色检测两组心肌细胞特异性标志物cTnI及Desmin的表达。结果 hMSCs经正常搏动CMs上清诱导培养后，只有少数细胞变宽大，部分细胞表达cTnI，但不表达Desmin；经损伤CMs上清诱导培养后，hMSCs变为宽大，大部分细胞表达cTnI，部分细胞表达Desmin。定量分析，正常与损伤状态下培养上清液诱导细胞表达cTnI、desmin的阳性百分比及阳性表达强度OD值均有非常显著性差异（P<0.01）。结论 正常与损伤状态下CMs培养上清液均可诱导hMSCs向心肌样细胞分化，CMs损伤状态下的细胞培养上清液更有利于促进其分化。

关键词: 人骨髓间充质干细胞(hMSCs), 心肌样细胞, 细胞分化, cTnI, Desmin

Abstract: Objective To observe whether human bone marrow mesenchymal stem cells (hMSCs) could differentiate into cardiomyo-like cells by culturing with supernatant of normal or injured rat cardiomyocytes (CMs) in vitro. Methods hMSCs were cultured with supernatant from normal or injured rat CMs for 27~30 d. The morphologies of induced hMSCs were observed with inverted microscope and the special cardio-markers cTnI and Desmin were identified with immunocytochemisry. Results A few cells cultured with supernatant from normal CMs enlarged and expressed cTnI, but little Desmin. While more cells cultured with supernatant from injured CMs enlarged and expressed cTnI, and parts of them expressed Desmin. The incidence of cTnI or Desmin positive cells were significantly different between these two groups (P<0.01). Conclusion Supernatant from both normal and injured CMs can induce hMSCs into cardio-like cells in vitro, and that from injured CMs is more effectively.

Key words: human bone marrow mesenchymal stem cells (hMSCs), cardiomyo-like cells, differentiation, cTnI, Desmin