《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (03): 208-210.

• 专题 • 上一篇    下一篇

电针对急性脊髓损伤大鼠白细胞介素-1β表达变化的影响

孙为增1;王新家1;林丽艳2   

  1. 1. 汕头大学医学院附属第二医院关节脊柱外科,广东汕头市 515041;2. 汕头大学医学院病理生理教研室，广东汕头市 515041
  • 收稿日期:2008-12-31 出版日期:2009-03-01 发布日期:2009-03-01
  • 通讯作者: 王新家

Effect of Electroacupuncture on Expression of Interleukin-1β in Rats with Acute Spinal Cord Injury

SUN Wei-zeng, WANG Xin-jia, LIN Li-yan   

  1. Department of Spine Surgery, the Second Affiliated Hospital, Medical College of Shantou University, Shantou 515041, Guangdong, China
  • Received:2008-12-31 Published:2009-03-01 Online:2009-03-01

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被引次数

23

摘要： 目的 观察电针治疗对脊髓损伤(SCI)大鼠脊髓组织白细胞介素（IL）-1β表达变化的影响，探究其与脊髓损伤后炎症反应和细胞凋亡的关系。方法 选用2月龄SD大鼠80只。随机将大鼠分为对照组和电针组。采用Allen法建立大鼠急性SCI模型，用免疫组化方法检测两组大鼠脊髓神经元胞浆IL-1β及胱氨酸酶(caspase-3)变化，用比色法检测其脊髓组织匀浆超氧化物歧化酶（SOD）、丙二醛（MDA）水平，用原位末端标记法(TUNEL)检测两组大鼠脊髓神经元和胶质细胞凋亡。结果 治疗结束时，与对照组相比，电针组IL-1β和caspase-3阳性细胞数明显减少(P<0.01)，IL-1β和caspase-3阳性细胞的平均灰度值明显提高(P<0.01)；MDA水平明显降低(P<0.01)，SOD水平明显升高(P<0.01)；TUNEL阳性细胞数明显减少(P<0.01)。结论 电针治疗后，急性脊髓损伤大鼠脊髓IL-1β及caspase-3表达减少，减轻了脊髓损伤后炎症反应和细胞凋亡。

关键词: 电针, 针刺, 脊髓损伤, 白细胞介素-1β, 超氧化物歧化酶（SOD）, 丙二醛（MDA）, 脂质过氧化, 凋亡, 大鼠

Abstract: Objective To observe the changes of interleukin-1β (IL-1β) in spinal tissues of rats with acute spinal cord injury after electroacupuncture (EA) treatment, and its association with inflammatory reaction and apoptosis after spinal cord injury. Methods The spinal cord injury of all the rats was induced by a 10 g × 2.5 cm impact with the reformative Allen equipment. The IL-1β and caspase-3 in spinal nerves of rats were detected with immunohistochemistry, while the level of superoxide dismutase (SOD) and malondialdehyde (MDA) in spinal tissues with the Colourimetry, neuron apoptosis with the terminal deoxynucleotidyl transferase-mediated deoxyuredine triphosphate-biotin nick end labeling (TUNEL). Results At the end of EA therapy, the expression of IL-1β and caspase-3, the level of MDA, and the number of TUNEL positive cells in EA group were all significantly lower than those in the control group (P<0.01), and the activity of SOD was obviously higher (P<0.01). Conclusion The EA therapy can decrease the expression of IL-1β and caspase-3 in rats of acute spinal cord injury, and alleviate the inflammatory reaction and apoptosis after spinal cord injury.

Key words: electroacupuncture, acupuncture, spinal cord injury, interleukin-1β (IL-1β), superoxide dismutase (SOD), malondialdehyde (MDA), lipid peroxidation, apoptosis, rats