《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (03): 201-202.

• 专题 •    下一篇

莫诺苷抑制过氧化氢诱导的SH-SY5Y神经细胞钙超载和细胞毒

王文1;孙芳玲1;安宜2;黄文婷2;艾厚喜1;张丽1;李林1   

  1. 1.首都医科大学宣武医院药物研究室，教育部神经变性病学重点实验室，北京市 100053;2.北京理工大学生命科学与技术学院生物技术系，北京市 100081
  • 收稿日期:2008-12-12 出版日期:2009-03-01 发布日期:2009-03-01
  • 通讯作者: 王文、安宜

Morroniside Inhibits Hydrogen Peroxide-induced Calcium Overload and Cytotoxicity in SH-SY5Y Neuroblastoma Cells

WANG Wen, SUN Fang-ling, AN Yi, et al   

  1. Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Disease Ministry of Education， Beijing 100053, China
  • Received:2008-12-12 Published:2009-03-01 Online:2009-03-01

PDF (PC)

被引次数

56

摘要： 目的 研究莫诺苷抑制过氧化氢诱导的SH-SY5Y神经细胞钙超载和细胞毒作用。方法 体外培养的人神经母细胞瘤细胞系SH-SY5Y细胞分别加入莫诺苷1 μmol/L、10 μmol/L、100 μmol/L预孵育24 h，加入H2O2 300~500 μmol/L，作用18 h，检测细胞内游离钙离子浓度、乳酸脱氢酶(LDH)释放率。结果 与未加莫诺苷预孵育的细胞相比，莫诺苷10 μmol/L、100 μmol/L能抑制神经细胞内游离钙离子浓度增加，莫诺苷1 μmol/L、10 μmol/L、100 μmol/L能降低LDH的释放率。结论 莫诺苷能够抑制H2O2诱导的SH-SY5Y神经细胞钙超载和细胞毒，有神经保护作用。

关键词: 莫诺苷, 人神经母细胞瘤细胞, SH-SY5Y系, 氧化应激, 钙超载, 细胞毒

Abstract: Objective To investigate the effects of morroniside on hydrogen peroxide (H2O2)-induced calcium overload and cytotoxicity in SH-SY5Y neuroblastoma cells. Methods SH-SY5Y cells were pre-incubated with morroniside 1, 10, or 100 μmol/L for 24 h prior to exposure to H2O2 300~500 μmol/L for 18 h. The cytosolic free calcium concentration and LDH release were determined. Results The H2O2-induced cytosolic free calcium concentration decreased in the cells pre-incubated with morroniside 10 or 100 μmol/L, while the LDH release level decreased in the cells pre-incubated with morroniside 1, 10 or 100 μmol/L, comparison with the cells exposed H2O2 along. Conclusion Morroniside effects neuroprotection against H2O2-induced calcium overload and cytotoxicity in SH-SY5Y cell.

Key words: Morroniside, neuroblastoma cell, SH-SY5Y, oxidative stress, calcium overload, cytotoxicity