《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (4): 310-312.

• 论文 • 上一篇    下一篇

缺氧缺血性脑损伤后针康法对幼鼠学习记忆能力及海马微管相关蛋白-2表达的影响

孔妍1，唐强1,2，朱路文1，刘波2   

  1. 1.黑龙江中医药大学，黑龙江哈尔滨市 150040；2.黑龙江中医药大学附属二院康复医学科，黑龙江哈尔滨市 150001。
  • 收稿日期:2011-03-16 修回日期:1900-01-01 出版日期:2011-04-25 发布日期:2011-04-25
  • 通讯作者: 唐强

Effect of Cluster Needling of Scalp Acupuncture Combined with Rehabilitation on Learning and Memory and Expression of Hippocampus Microtubule-associated Protein-2 in Young Rats after Hypoxic Ischemic Brain Damage

KONG Yan, TANG Qiang, ZHU Lu-wen, et al.   

  1. Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, Heilongjiang, China
  • Received:2011-03-16 Revised:1900-01-01 Published:2011-04-25 Online:2011-04-25

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被引次数

37

摘要： 目的探讨针康法对缺氧缺血性脑损伤(HIBD)幼鼠学习记忆能力及海马微管相关蛋白-2(MAP-2)的影响。方法将80只Wistar幼鼠随机分为5组：假手术组、模型组、头穴丛刺组、环境刺激组和针康组，每组16只。每组再按造模后14 d、28 d分别处死分为2个亚组，每组8只。采用结扎左侧颈总动脉，吸入氧体积浓度为8%的氧氮混合气体，制作HIBD新生动物模型。采用Morris水迷宫评价幼鼠学习、记忆能力，免疫组化法检测海马MAP-2阳性细胞的表达。结果水迷宫实验结果显示：模型组逃避潜伏期时间延长，穿台次数减少。在定位航行、空间探索能力方面头穴丛刺组、环境刺激组和针康组与模型组比较，差异有统计学意义(P＜0.05)。在定位航行、空间探索能力方面针康组分别与头穴丛刺组、环境刺激组比较有统计学意义(P＜0.05)。免疫组化检结果显示：头穴丛刺组、环境刺激组、针康组海马CA1区MAP-2的表达均多于模型组(P＜0.05)，海马CA1区MAP-2表达针康组较头穴丛刺组、环境刺激组表达增加(P＜0.05)。结论针康法能提高HIBD幼鼠学习记忆能力，其分子机制可能与海马CA1区MAP-2阳性细胞的表达水平增加有关。

关键词: 缺氧缺血性脑损伤, 针康法, 微管相关蛋白-2, 大鼠

Abstract: ObjectiveTo observe the effect of cluster needling of scalp acupuncture combined with rehabilitation(Tang's Approach) on learning and memory and the expression of hippocampus microtubule-associated protein-2 (MAP-2) in young rats after hypoxic ischemic brain damage (HIBD).Methods80 Wistar young rats were randomly divided into 5 groups: sham group, model group, scalp cluster acupuncture group, environmental stimuli group, and acupuncture with rehabilitation group, 16 rats in each group. Each group was divided into 2 subgroups on the 14th and 28th days after the model. HIBD young animal model was made by ligating left common carotid artery and inspiring 8% oxygen and 92% nitrogen mixture. Learning and memory was observed by Morris water maze, and MAP was observed by immunohistochemistry.ResultsThe results of the Morris water maze test showed that there was significant difference in ability of place navigation and space exploration in the scalp cluster acupuncture group, the environmental stimuli group, and the acupuncture with rehabilitation group compared with the model group (P<0.05), and there was significant difference in ability of place navigation and space exploration in the acupuncture with rehabilitation group compared with the scalp cluster acupuncture group and the environmental stimuli group (P＜0.05). The results of immunohistochemistry showed that there was significant difference in the expression of MAP-2 in the scalp cluster acupuncture group, the environmental stimuli group, and the acupuncture with rehabilitation group compared with the model group (P<0.05), and there was significant difference in the expression of MAP-2 in the acupuncture with rehabilitation group compared with the scalp cluster acupuncture group and the environmental stimuli group (P＜0.05). ConclusionLearning and memory of young rats after hypoxic ischemic brain damage can be improved by Tang's Approach which is possibly related with expression of MAP-2 in hippocampus.

Key words: hypoxic ischemic brain injury, Tang's Approach, microtubule-associated protein-2, rats