《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (4): 329-333.

• 论文 • 上一篇    下一篇

壳聚糖-碱性成纤维细胞生长因子载体诱导骨髓间充质干细胞向神经细胞分化

段红梅，杨朝阳，李晓光   

  1. 首都医科大学神经科学研究所，北京市 100069。
  • 收稿日期:2011-01-27 修回日期:2011-04-11 出版日期:2011-04-25 发布日期:2011-04-25
  • 通讯作者: 李晓光

Basic Fibroblast Growth Factor-chitosan Carriers Induce Bone Marrow-Derived Mesenchymal Stem Cells to Differentiate into Nerve Cells

DUAN Hong-mei, YANG Zhao-yang,LI Xiao-guang.   

  1. Beijing Institute for Neuroscience, Capital Medical University, Beijing 100069, China
  • Received:2011-01-27 Revised:2011-04-11 Published:2011-04-25 Online:2011-04-25

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被引次数

14

摘要： 目的探索壳聚糖-碱性成纤维细胞生长因子（bFGF）载体对骨髓间充质干细胞(MSCs)向神经细胞分化的诱导作用。方法免疫组织化学、Western blot检测MSCs诱导分化为神经细胞，MTT检测诱导后细胞的活性。结果MSCs经壳聚糖-bFGF载体诱导后，表达神经干细胞的标记物Nestin以及神经细胞的标记物β-tubulin Ⅲ和MAP-2，比例高达83.54%。结论壳聚糖-bFGF载体可以诱导MSCs高比例向神经细胞分化。

关键词: 骨髓间充质干细胞, 碱性成纤维细胞生长因子, 壳聚糖, 诱导, 神经分化, 细胞因子, 神经细胞

Abstract: ObjectiveTo explore the effect of bFGF-chitosan carriers on inducing bone marrow-derived mesenchymal stem cells (MSCs) to differentiate into nerve cells.MethodsMSCs were detected by immunohistochemistry and Western blot after they were induced by bFGF-chitosan carriers to differentiate into neurons. The MTT chromometry assay was carried out to determine cell viability.ResultsThe proportion of express neural stem cells marker Nestin, and neuronal markers class Ⅲ β-tubulin and MAP-2 was 83.54% after MSCs induced by bFGF-chitosan carriers.ConclusionbFGF-chitosan carriers can induce MSCs to differentiate into nerve cells with a high percentage.

Key words: bone marrow-derived mesenchymal stem cells, basic Fibroblast Growth Factor, chitosan, induction, neural differentiation, cytokine, nerve cells