《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (12): 1104-1107.

• 专题 • 上一篇    下一篇

蛇毒神经生长因子对大鼠脑缺血再灌注损伤后神经细胞GAP-43表达的影响

石胜良1;张跃龄1;陈仕检1;毕桂南1;黎彬如2;刘唐威1   

  1. 1.广西医科大学第一附属医院神经内科，广西南宁市 530021;2.广西壮族自治区民族医院神经内科，广西南宁市 530021
  • 收稿日期:2009-10-29 出版日期:2009-12-25 发布日期:2009-12-25
  • 通讯作者: 刘唐威

Effects of Venom Nerve Growth Factor on GAP-43 of Nerve Cell Following Cerebal Ischemia/reperfusion in Rat

SHI Sheng-liang,ZHANG Yue-ling,CHEN Shi-jian,et al   

  1. Department of Neurology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China
  • Received:2009-10-29 Published:2009-12-25 Online:2009-12-25

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摘要： 目的 探讨蛇毒神经生长因子(vNGF)对大鼠脑缺血再灌注的保护作用。方法 选用体重220～280 g的健康雄性Wistar大鼠，分为假手术组、对照组及vNGF 组（再分为25 U、50 U、100 U 3个剂量组）。所有大鼠侧脑室注药7 d后处死。大鼠模型分别观察：按Longa法进行神经功能评分；免疫组化法检测GAP-43的表达。结果 神经功能评分：假手术组神经功能评分为0分，其余各组均出现神经功能缺损，vNGF组大鼠评分低于对照组（P<0.05）。免疫组化：除假手术组外各组GAP-43均有表达。vNGF组的表达较对照组高（P<0.05）。vNGF组25 U组GAP-43表达增加，50 U组表达继续增高，100 U组表达最高。结论 在大鼠大脑中动脉缺血再灌注损伤后，vNGF能增强GAP-43的表达，对神经细胞有明显的保护作用。

关键词: 大鼠, 脑缺血再灌注, 蛇毒神经生长因子, 神经细胞生长相关蛋白-43(GAP-43)

Abstract: Objective To investigate the cerebral ischemia/reperfusion protection mechanism of viper venom nerve growth factor(vNGF) by the change of expression of growth associated protein-43 (GAP-43) and neurological function.Methods 45 adult male Wistar rats (weight 220～280 g) were divided randomly into 3 groups: sham group(S, n=9), balanced salt solution group (BSS, n=9) and venom nerve growth factor group (vNGF, n=27). Each group was observed for 7 days. vNGF group was divided into 25 U, 50 U and 100 U subgroups respectively. The following indexes in 3 groups were observed respectively: neurologic deficits and the expression of GAP-43 (immunohistochemistry method).Results Neurological function: The scores of neurological function was 0 in S group. The neurological deficits score was lower at the same time in vNGF group than that in BSS group (P<0.05). Immunohistochemistry: GAP-43 expressed in both BSS group and vNGF group. The expression of GAP-43 in vNGF group increased in 25 U, and to maximum in 100 U. The expression of GAP-43 in BSS group was significantly lower than in vNGF group (P<0.05). Conclusion vNGF can effectively enhance and prolong the expression of GAP-43, increase the survival rats of nerve cells, and has the protection effect on nerve cells after cerebral ischemia injured.

Key words: rat, cerebal ischemia/reperfusion, viper venom nerve growth factor, growth associated protein-43