《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (09): 835-837.

• 基础研究 • 上一篇    下一篇

丝裂原激活的蛋白激酶在脑缺血预适应中的作用：ERK、JNK和p38作用的比较

赵兰峰;郭社卫;安仰原;刘明刚   

  1. 首都医科大学基础医学院神经生物学系疼痛生物医学研究所，北京市 100069
  • 收稿日期:2009-06-03 出版日期:2009-09-01 发布日期:2009-09-01

Roles of Mitogen Activated Protein Kinases in Cerebral Ischemic Preconditioning: Comparison among ERK, JNK and p38

ZHAO Lan-feng,GUO She-wei,AN Yang-yuan,et al   

  1. Institute for Biomedical Sciences of Pain, Department of Neurobiology, Capital Medical University School of Basic Medical Sciences, Beijing 100069, China
  • Received:2009-06-03 Published:2009-09-01 Online:2009-09-01

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摘要： 目的 初步探讨丝裂原激活的蛋白激酶(MAPKs)3条主要信号通路在大鼠脑缺血预适应中（IPC）的作用。方法 将大鼠随机分为正常对照（control）、假手术(SH)、缺血预适应/缺血耐受(IT)3组，每组6只。应用SDS-PAGE、Western blot等实验方法，借助Gel Doc和Versa Doc凝胶成像系统，半定量检测大鼠大脑躯体感觉皮层ERK、JNK 、p38磷酸化水平和蛋白表达量的变化。结果 IT组大鼠躯体感觉皮层内ERK1和JNK46KD的磷酸化水平增高（P<0.05）。结论 大鼠躯体感觉皮层内MAPKs信号通路中ERK1和JNK46KD激活可能参与了脑缺血预适应的形成，但也不能排除p38在其中的作用。

关键词: 脑缺血预适应, 丝裂原激活的蛋白激酶(MAPKs), 磷酸化, 蛋白表达

Abstract: Objective To explore initially the roles of the 3 major signaling pathways of mitogen activated protein kinases (MAPK) in cerebral ischemia preconditioning. Methods Healthy adult SD rats were randomly divided into 3 groups: normal control group; sham-operated group and ischemic preconditioning or ischemic tolerance group (n=6). SDS-PAGE, Western blot and Gel Doc imagine systems were applied to determine the phosphorylation and protein expression of ERK, JNK and p38 in somatosensory cortex of rat. Results The phosphorylation level of ERK1 and JNK46KD in somatosensory cortex increased significantly (P<0.05) after ischemia preconditioning. Conclusion The increased ERK1 and JNK46KD phosphorylation in somatosensory cortex may be involved in the development of cerebral ischemia preconditioning and can not be ruled out in which the role of p38.

Key words: cerebral ischemic preconditioning, mitogen activated protein kinases (MAPK), phosphorylation, protein expression