《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (12): 1101-1103.

• 专题 •    下一篇

莫诺苷对大鼠脑缺血再灌注神经凋亡的影响

王文1;许栋明2;相婕1;李蕾1;王培昌1;艾厚喜1;张丽1;李林1   

  1. 1.首都医科大学宣武医院药物研究室，北京市 100053;2.科技部火炬高技术产业开发中心，北京市 100038
  • 收稿日期:2009-11-12 修回日期:1900-01-01 出版日期:2009-12-25 发布日期:2009-12-25

Effects of Morroniside on Apoptosis in Rats with Focal Cerebral Ischemia/Reperfusion Injury

WANG Wen,XU Dong-Ming,XIANG Jie,et al   

  1. Xuanwu Hospital of Capital Medical University, Beijing 100053; Torch High Technology Industry Development Center, The Ministry of Science and Technology of the P.R.C., Beijing 100038, China
  • Received:2009-11-12 Revised:1900-01-01 Published:2009-12-25 Online:2009-12-25

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摘要： 目的 研究山茱萸有效成分莫诺苷对局灶性脑缺血再灌注大鼠皮层谷胱甘肽含量及Caspase-3的影响。方法 用线栓法制备大鼠局灶性脑缺血再灌注模型，缺血30 min，再灌注7 d。采用分光光度法检测大鼠皮层谷胱甘肽含量；用Western blot方法检测Caspase-3表达。结果 莫诺苷(270 mg/kg)能显著增加大鼠谷胱甘肽含量，降低Caspase-3表达。结论 莫诺苷能通过增强大鼠皮层抗氧化能力而抑制脑缺血再灌注引起的神经元凋亡。

关键词: 莫诺苷, 缺血再灌注, 谷胱甘肽, 细胞凋亡, 神经保护

Abstract: Objective To investigate the effects of morroniside on glutathione (GSH) and Caspase-3 in rats with focal cerebral ischemia/reperfusion. Methods The animal model was induced by middle cerebral artery occlusion with suture embolus with 30 min for cerebral ischemia and 7 d for reperfusion. The content of GSH was detected with spectrophotometry and Caspase-3 expression was observed by Western blot.Results Compared with model group, the GSH increased and Caspase-3 expression reduced significantly at 270 mg/kg of morroniside.Conclusion Morroniside may have neuroprotective effect by increasing GSH in rats cortex and reduce the apoptosis in focal cerebral ischemia/reperfusion injury.

Key words: morroniside, cerebral ischemia/reperfusion, glutathione, apoptosis, neuroprotective