《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (11): 1019-1020.

• 基础研究 • 上一篇    下一篇

裸鼠皮下脑胶质瘤模型的复制与生长特性的观察

潘静坤1;赵卉2;田磊1;罗芸1;薛毅珑1   

  1. 1.解放军总医院老年医学研究所细胞生物学研究室，北京市 100853;2. 解放军总参谋部管理保障部中心门诊部，北京市 100034
  • 收稿日期:2009-03-05 出版日期:2009-11-01 发布日期:2009-11-01
  • 通讯作者: 薛毅珑

Hypodermic of Glioma Cells into Nude Mice Inducing Glioma Model: Growth Characteristics Observation

PAN Jing-kun,ZHAO Hui,TIAN Lei,et al   

  1. Department of Geriatrics, Cell Biology Lab, Chinese PLA General Hospital, Beijing 100853, China
  • Received:2009-03-05 Published:2009-11-01 Online:2009-11-01

PDF (PC)

被引次数

2

摘要： 目的 建立裸鼠皮下C6脑胶质瘤模型，了解其生长特性。方法 将C6脑胶质细胞的培养悬液注射于裸鼠左侧腋下区皮下。观察接种区皮下肿瘤的生长状况，测量肿瘤直径并计算肿瘤体积，绘制肿瘤体积-时间生长曲线。HE染色和胶质纤维酸性蛋白(GFAP)免疫组化染色观察肿瘤细胞的生长及转移状况。结果 瘤细胞接种后第7天，裸鼠腋下区开始出现肿瘤，并呈加速生长。20 d后，皮下肿瘤呈快速的近指数生长。裸鼠生存期为22～48 d。光镜下见瘤体边界清晰，瘤内血管丰富，存在大量GFAP阳性细胞。结论 将体外培养的C6脑胶质瘤细胞植入裸鼠皮下，可建立重复性好的脑胶质瘤动物模型。

关键词: 动物模型, 脑胶质瘤, 皮下接种, 裸鼠

Abstract: Objective To establish the model of glioma by hypodermic of C6 cells line into the nude mice and observe the growth characteristics. Methods The C6 gliocyte suspension was injected into the left subaxillary hypoderma of nude mice. The diameter and the volume of the tumor were measured and calculated, and the volume-time growth curve was plotted. The cells of tumor were observed under the HE and glial fibrillary acidic protein (GFAP) immono-histochemistry staining. Results The tumor appeared in inoculation area 7 d after injection, and presented nearly exponential development after about 20 d. The survival time of the nude mice is 22～48 d. The tumors were with sharp border, affluent blood vessel, and a great number of GFAP-positive cells. Conclusion The model of glioma can be well induced by hypodermic of the cultured C6 gliocyte into nude mouse.

Key words: animal model, glioma, hypodermic inoculation, nude mouse