《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (10): 973-976.

• 论文 • 上一篇    下一篇

针康法对局灶性脑缺血大鼠前肢运动功能及缺血区突触素和生长相关蛋白-43 表达的影响

唐强1，刘宏光2，王艳1，朱路文2，李洪梅2   

  1. 1.黑龙江中医药大学附属第二医院，黑龙江哈尔滨市150001。2.黑龙江中医药大学，黑龙江哈尔滨市150040。
  • 收稿日期:2011-07-07 修回日期:2011-08-19 出版日期:2011-10-25 发布日期:2011-10-25

Effects of Cluster Needling of Scalp Acupuncture Combined with Rehabilitation on Forelimb Motor Function and Expression of Synaptophysin and Growth-associated Protein-43 in Rats after Focal Cerebral Ischemia

TANG Qiang, LIU Hong-guang, WANG Yan,et al.   

  1. The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150001, Heilongjiang, China
  • Received:2011-07-07 Revised:2011-08-19 Published:2011-10-25 Online:2011-10-25

PDF (PC)

摘要： 目的观察针康法对局灶性脑缺血大鼠前肢运动功能及缺血区周围皮质突触素和相关蛋白-43(GAP-43)表达的影响。方法采用内皮素-1(ET-1)诱导局灶性脑缺血大鼠前肢损伤模型。将大鼠随机分为5 组：假手术组、模型组、康复组、针刺组、针康组，每组又分为3 d、7 d、14 d、21 d 4 个亚组。造模后24 h 对针康组进行头穴丛刺结合前肢抓取训练，康复组给予前肢抓取训练，针刺组仅头穴丛刺法，模型组不给予任何干预。分析前肢抓取成功率及应用免疫组化方法观察各组不同时间点缺血灶周围皮质突触素和GAP-43 表达。结果造模后各时间点，针康组大鼠前肢抓取成功率优于模型组、康复组和针刺组(P<0.05)。针康组缺血区周围皮质突触素平均光密度在造模后14 d 和21 d 优于模型组、康复组和针刺组(P<0.05)；针康组缺血区周围皮质GAP-43 阳性细胞数量在造模后7 d 和14 d 优于模型组、康复组和针刺组(P<0.05)。结论针康法可促进局灶性脑缺血大鼠前肢运动功能的恢复，其机制可能与增强脑缺血区周围皮质突触素和GAP-43 的表达有关。

关键词: 针康法, 脑缺血, 前肢运动功能, 突触素, 生长相关蛋白-43, 大鼠

Abstract: Objective To explore the effects of cluster needling of scalp acupuncture combined with rehabilitation (Tang's Approach) onforelimb motor function and expression of synaptophysin and growth-associated protein (GAP-43) in rats after focal cerebral ischemia.Methods Endothelin-1(ET-1) was used to induce focal cerebral ischemia model in rats with forelimb dysfunction. The rats were randomlydivided into 5 groups: sham operation group, model group, rehabilitation group, acupuncture group, and acupuncture with rehabilitationgroup, each group was divided into 4 subgroups by 3 d, 7 d, 14 d, and 21 d. 24 hours after modeling, the cluster needle of scalp point withskilled forelimb reaching was trained in the acupuncture with rehabilitation group, skilled forelimb reaching was trained in the rehabilitationgroup, and the cluster needle of scalp point only were punctured and stimulated in the acupuncture group. But the model group had no intervention.The success rates of forelimb reaching were analyzed and immuno-histochemical technique was used to observe the expression ofsynaptophysin and GAP-43 in cortical ischaemic lesions of the rats in each group at different time points. Results At different time pointsafter the treatment, the successful rate of forelimb reaching in the acupuncture with rehabilitation group was higher than the model group, rehabilitationgroup, and acupuncture group (P<0.05). Conclusion The cluster needling of scalp acupuncture combined with rehabilitationcan promote recovery of forelimb motor function in rats after focal cerebral ischemia, which may associate with the expression of synaptophysinand GAP-43 in cortex surro`unding following focal cortical ischaemic lesions.

Key words: Tang's Approach, cerebral ischemia, forelimb motor function, synaptophysin, growth-associated protein-43, rats