《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (10): 942-944.

• 基础研究 • 上一篇    下一篇

析因设计在针灸治疗脊髓损伤康复研究中的应用

高莉敏1;孙迎春2   

  1. 1.北京中医药大学管理学院，北京市 100029;2.首都医科大学康复医学院，北京市 100068
  • 收稿日期:2009-04-21 出版日期:2009-10-01 发布日期:2009-10-01

Application of Factorial Design in Study of Intervention Effects of Acupuncture on Spinal Cord Injury Rats

GAO Li-min,SUN Ying-chun   

  1. School of Administration, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2009-04-21 Published:2009-10-01 Online:2009-10-01

PDF (PC)

被引次数

9

摘要： 目的 运用析因设计方法，研究电针刺激对脊髓损伤的治疗效果，探讨不同针灸部位（穴位）及时间对脊髓损伤康复的联合干预作用，为临床针灸治疗脊髓损伤提供理论依据。方法 拟定析因设计中的因素与水平，将96只脊髓损伤大鼠随机分配到实施运动区头皮表面投影电针刺激组（A组，即头针）、局部电针刺激组（B组，即体针）、头针体针联合干预组（A+B组）、未干预组（D组，对照）。对各组受试对象观测不同时间（第1周、第2周、第4周、第8周、第12周）的BBB评分及运动诱发电位MEP潜伏期及波幅，进行2 ×2 ×5析因分析。结果 单纯使用头针或体针均能使模型鼠运动BBB评分、运动诱发电位MEP波幅提高，缩短MEP潜伏期(P＜0.05)；使用头针、 体针对BBB评分、运动诱发电位MEP潜伏期及波幅存在交互作用(P＜0.05)，提示头针与体针联合干预对促进脊髓损伤的康复效果更佳。各组随着针灸干预的时间增加表现出不同程度的BBB评分、MEP波幅提高及潜伏期缩短，时间因素5个水平均数之间有统计学意义(P＜0.05)。结论 析因设计方法可应用于针灸疗效评价中多因素多水平的研究。

关键词: 析因设计, 针灸, 电针刺激, 脊髓损伤

Abstract: Objective To study and observe the intervention effects of Acupuncture on spinal cord injury rats by using factorial design. The best combination rules of the best time course and electrical stimulation site were explored.Methods 96 experimental spinal cord injury rats were randomly divided into four groups: acupuncture on head intervention group (group A), acupuncture on body intervention group (group B), complex intervention group (group A+B) and nonintervention group (group D) . There were 24 rats in each group. The rats received electrical stimulation according experimental design. The motor function of the rats' hind limbs were detected at different time sites(1 w, 2 w, 4 w, 8 w and 12 w).Results The Results among different Methods of electrical stimulation are significantly different. The levels of the rat's BBB scale, and evoked potential(MEP) in the group A+B were the best among four groups (P＜0.05). The BBB scale and evoked potential(MEP)of the rats in each group are significantly different at different time sites (P＜0.05). It means that each kind of electrical stimulation plays a positive role in promoting restoration of motor function. The correct analysis for factorial design data is helpful to the fully application of information.Conclusion Factorial design can be used in research projects of Acupuncture, especially the design for multiple factors and multiple levels.

Key words: factorial design, acupuncture, electrical stimulation, spinal cord injury