《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (03): 163-164.

• 基础研究 • 上一篇    下一篇

鸦胆子油乳对膀胱癌细胞系BIU-87坏死与凋亡的影响

李晓武1; 王禾1; 秦卫军1; 李欣1; 刘涛3; 樊春梅2   

  1. 1.第四军医大学西京医院泌尿外科 陕西西安市 710033;2.第四军医大学西京医院临床免疫科 陕西西安市 710033;3.第四军医大学基础部解剖教研室 陕西西安市 710033
  • 收稿日期:2004-01-25 出版日期:2004-03-25 发布日期:2004-03-25

Necrosis and apoptosis induced by brucea javanica oil emulsion in bladder cancer cell

LI Xiao-wu, WANG He, QIN Wei-jun, et al   

  1. Department of Urology Surgery, XijingHospital, Fourth Military Medical University, Xi’an 710033, Shanxi, China
  • Received:2004-01-25 Published:2004-03-25 Online:2004-03-25

PDF (PC)

被引次数

59

摘要： 目的探讨鸦胆子油乳对膀胱癌BIU-87细胞坏死与凋亡的影响及其可能的作用机制。方法将不同浓度的鸦胆子油乳加入体外培养的BIU-87细胞,用流式细胞仪检测细胞凋亡与坏死比例、并检测线粒体膜电位(MMP),用激光共聚焦显微镜观察细胞色素C分布。结果高浓度鸦胆子油乳作用4h后,肿瘤细胞MMP明显降低,24h后多数细胞发生坏死,与对照组相比有非常显著性差异(P<0.01);低浓度鸦胆子油乳作用6h后,诱导细胞色素C释放,24h后可见典型的凋亡现象,与对照组相比有显著性差异(P<0.05)。结论鸦胆子油乳高浓度主要诱导肿瘤细胞坏死,低浓度诱导细胞凋亡。

关键词: 鸦胆子, 膀胱肿瘤, 坏死, 凋亡, 线粒体, 细胞色素C

Abstract: Objective To study the effcet of necrosis and apoptosis induced by Brucea javanica oil emulsion on bladder cancer BIU-87 cells. MethodsBrucea javanica oil emulsion of different concentration was added to the bladder cancer BIU-87 cell cultured in vitro. The ratio of apoptosis/necrosis cells and mitochondrial membrane potential(MMP) were detected with flow cytometer. The dissipation of cytochrome C was observed with confocal microscopy. ResultsHigh concentration brucea javanica oil emulsion reduced MMP which led cell necrosis, while that of low concentration diffused the cytochrome C from mitochondria to cytoplasm which induced cell apoptosis. ConclusionHigh concentration brucea javanica oil emulsion mainly causes BIU-87 cells to necrosis, low concentration induces cell to apoptosis.

Key words: brucea javanica, bladder neoplasm, necrosis, apoptosis, mitochondria, cytochrome C