《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (08): 717-719.

• 专题 • 上一篇    下一篇

电针刺激对脊髓损伤大鼠植入脐血干细胞后生长相关蛋白、碱性成纤维细胞生长因子、胰岛素样生长因子表达的影响

孙迎春1,2;李建军1,2;高莉敏3;季凤清4   

  1. 1.首都医科大学康复医学院，北京市 100068;2.中国康复研究中心北京博爱医院，北京市 100068;3.北京中医药大学管理学院，北京市 100029;4.首都医科大学，北京市 100069
  • 收稿日期:2009-06-16 出版日期:2009-08-01 发布日期:2009-08-01

Effect of Electrical Stimulation on Expression of Growth Associated Protein-43, basic Fibroblast Growth Factor, Insulin-like Growth Factor in Spinal Cord Injured Rats after Human Umbilical Cord Blood Stem Cells Transplantation

SUN Ying-chun,LI Jian-jun,GAO Li-min,et al   

  1. Capital Medical University School of Rehabilitation Medicine, Beijing Charity Hospital, China Rehabilitation Research Centre, Beijing 100068, China
  • Received:2009-06-16 Published:2009-08-01 Online:2009-08-01

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摘要： 目的 探讨不同电针刺激方案对大鼠脊髓损伤植入脐血干细胞后生长相关蛋白（GAP-43）、碱性成纤维细胞生长因子（bFGF）、胰岛素样生长因子（IGF）表达的影响。方法 将192只清洁级SD大鼠用NYU打击器制成T10-11段脊髓损伤模型，造模成功后随机分为：A组为运动区头皮表面投影区电刺激组(A1组电刺激+脐血干细胞移植，A2组只进行电刺激)；B组为损伤局部电刺激组（B1组电刺激+脐血干细胞移植，B2组只进行电刺激）；C组为运动区头皮表面投影区电刺激+局部电刺激组（C1组电刺激+脐血干细胞移植，C2组只进行电刺激）；D组为损伤模型组（D1组移植脐血干细胞不进行电刺激，D2组不移植不进行电刺激）。各组分别于1周、2周、3周、4周、8周、12周取材，采用免疫组织化学荧光染色检测生长相关蛋白（GAP-43）、碱性成纤维细胞生长因子（bFGF）、胰岛素样生长因子（IGF）的阳性表达。结果 在各时间点上，电刺激均能提高生长活性物质GAP-43、bFGF、IGF水平；头针加体针的影响大于单纯头针或体针；干细胞移植后水平更高。结论 电刺激与干细胞移植对脊髓损伤后微环境均有有利影响，并有协同作用；头针加体针优于单纯头针或体针。

关键词: 针灸, 电刺激, 干细胞移植, 脊髓损伤, 生长相关蛋白（GAP-43）, 碱性成纤维细胞生长因子（bFGF）, 胰岛素样生长因子（IGF）

Abstract: Objective To explore the effects of electrical stimulation in different acupoints on the expression of growth associated protein-43 (GAP-43), basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF) in spinal cord injured rats after the human umbilical cord blood stem cells transplantation.Methods 192 SD rats were injured at T10-11 level with NYU, and divided randomly into groups: Group A received electric stimulation in the scalp of motor area (A1 group received electric stimulation and transplantation of umbilical cord blood stem cell, the rats of A2 group only received electric stimulation); group B received local electric stimulation at damaged site (B1 with electric stimulation and transplantation, B2 only with electric stimulation); group C received electric stimulation in the scalp of motor area and at damaged site (C1 with electric stimulation and transplantation, C2 only with electric stimulation); D1 received transplantation without electric stimulation, D2 neither with transplantation nor electric stimulation. The expression of GAP-43, bFGF and IGF were detected 1, 2, 3, 4, 8 and 12 weeks after operation. Results Electric stimulation increased the expression of GAP-43, bFGF, IGF, stimulating scalp and body were more than stimulated scalp or body alone, combined with the stem cells transplantation were more than no transplantation. Conclusion Both electric stimulation and stem cells transplantation can improve the microenvironment for neural recovery synergistically. Stimulating scalp and body is more effective than alone.

Key words: acupuncture, electric stimulation, stem cells transplantation, spinal cord injury, growth associated protein-43 (GAP-43), basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF)