《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (8): 739-741.

• 论文 • 上一篇    下一篇

条斑紫菜多糖对糖尿病大鼠脑组织的保护作用

杨旭东，张杰，林峰   

  1. 牡丹江医学院药理教研室，黑龙江牡丹江市 157011。
  • 收稿日期:2011-04-01 修回日期:2011-06-20 出版日期:2011-08-25 发布日期:2011-08-25

Protecting Effect of Porphyra Yezoensis Polysaccharide on Cerebrum Tissue of Diabetic Rats

YANG Xu-dong, ZHANG Jie, LIN Feng.   

  1. Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang, China
  • Received:2011-04-01 Revised:2011-06-20 Published:2011-08-25 Online:2011-08-25

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摘要： 目的观察条斑紫菜多糖(PYP)对糖尿病大鼠脑组织的保护作用及对细胞凋亡的影响。方法将60 只Wistar 大鼠随机分为：正常对照组、模型组、PYP 高、中、低剂量组。HE 染色观察脑组织病理变化，琼脂糖凝胶电泳法检测细胞凋亡的情况，RT-PCR观察p53、bcl-2 基因mRNA的表达。结果PYP 可以明显改善糖尿病大鼠脑组织病理形态改变。与模型组相比，PYP 组大鼠脑组织凋亡细胞显著减少，p53 基因表达明显减少，bcl-2 基因表达明显增加。结论PYP 可以抑制糖尿病大鼠脑组织的p53 基因表达，促进bcl-2 基因表达，降低脑损伤。

关键词: 糖尿病, 条斑紫菜多糖, 神经病变, 细胞凋亡, p53 基因, bcl-2 基因

Abstract: Objective To observe the effect of porphyra yezoensis polysaccharide (PYP) on cerebrum tissue of diabetic rats and apoptosis. Methods 60 Wistar rats were randomly divided into normal control group, diabetic model group, and treated groups respectively with PYP (0.25 g/kg × d), PYP (0.5 g/kg × d), and PYP (1 g/kg × d). The cerebrum pathologic changes were observed by HE staining method, and the number of apoptotic cells was observed by electrophoresis and the mRNA expression of p53 and bcl-2 was confirmed by RT-PCR. Results PYP improved the cerebrum pathologic changes of diabetic rats. With comparison to the diabetic model group, the cerebrum pathologic change in the treated groups also improved. And the number of apoptotic cells, the expression of p53 in cerebrum tissue decreased and expressions of bcl-2 rose. Conclusion PYP can decrease the expression of p53 in cerebrum tissue and rise expressions of bcl-2 so as to protect brain tissues from cerebral ischemia.

Key words: diabetes mellitus, porphyra yezoensis polysaccharide, neuropathy, apoptosis, p53 gene, bcl-2 gene