《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (01): 23-25.

• 专题 • 上一篇    下一篇

大鼠脑损伤对移植人胚神经干细胞存活和分化的影响

张泽舜1;万虹2;历俊华2;翟晶2;韩富1;王忠诚2   

  1. 1.广东省中医院神经外科 广东广州市 510000;2.北京市神经外科研究所 北京市 100050
  • 收稿日期:2003-12-12 出版日期:2004-01-25 发布日期:2004-01-25

Effects of fluid percussion injury on survival and differentiation of human embryonic neural stem cells in rats

ZHANG Ze-shun, WAN Hong, LI Jun-hua, et al   

  1. Neurosurgery Department of Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, Guangdong, China
  • Received:2003-12-12 Published:2004-01-25 Online:2004-01-25

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摘要： 目的探讨大鼠脑液压冲击伤(FPI)后对植入的人胚神经干细胞(HNSCs)存活和分化的影响。方法取8周龄人胎儿大脑皮层细胞,体外培养获得神经干细胞(NSCs),巢蛋白免疫荧光染色;SD大鼠制作FPI模型,于伤后24h在损伤区移植标有5’溴脱氧脲嘧啶(BrdU)的HNSCs,1周和4周后处死大鼠,邻片行BrdU/微管相关蛋白-2(MAP-2)和BrdU/胶质纤维酸性蛋白(GFAP)免疫组织化学双染。结果HNSCs移植后1周可见BrdU阳性细胞向周围迁移,且BrdU/MAP-2双阳性细胞多于BrdU/GFAP双阳性细胞;移植后4周BrdU阳性细胞迁移的范围更广,但细胞数量明显减少,脉络丛和微血管中可见BrdU阳性细胞,BrdU/GFAP双阳性细胞多于BrdU/MAP-2双阳性细胞。结论HNSCs能存活于损伤区域,移植后逐渐分化为星形胶质细胞,且易被内皮吞噬细胞吞噬。

关键词: 液压冲击伤(FPI), 神经干细胞(NSC), 分化

Abstract: ObjectiveTo investigate the effects of fluid percussion injury(FPI) on survival and differentiation of transplanted human embryonic neural stem cells (HNSCs) in rats. MethodsThe HNSCs were separated from the cerebral cortex of the 8-week-old fetal and were cultured in DMEM/F12 combinated with EGF, bFGF and LIF. The rat models of FPI were made with fluid percussion system. The HNSCs labeled with BrdU were transplanted into the injured zone 24 hours after brain injury, then the rats were killed at the 1st and 4th week post-transplanted stages, and the brain slices were stained with immunocytochemistry. The GFAP, MAP-2, and BrdU positive cells were investigated.ResultsThe transplanted HNSCs migrated to the whole brain, and differentiated into GFAP and MAP-2 positive cells. MAP-2 positive cells were observed at 1 week post-transplanted stage, on the contrary, more GFAP positive cells were discovered 4 weeks after transplantation. Part of the HNSCs migrated to the choroids plexus of the lateral ventricle and microvessels. ConclusionThe transplanted HNSCs survive in the injured zone, and differentiate into astrocytes gradually during the recovery. The host devours part of the HNSCs.

Key words: fluid percussion injury (FPI), neural stem cells(NSCs), differentiation