《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (01): 17-18.

• 专题 • 上一篇    下一篇

神经组织细胞特异性蛋白在大鼠羊膜上皮细胞中的表达

孟晓婷1; 陈东1; 刘佳梅1; 路来金2   

  1. 1.吉林大学基础医学院组织学与胚胎学教研室 吉林长春市 130021;2.吉林大学第一临床学院 吉林长春市 130021
  • 收稿日期:2003-12-12 出版日期:2004-01-25 发布日期:2004-01-25

Expression of specific proteins of neural cell in amniotic epithelial cells in rats

MENG Xiao-ting, CHEN Dong, LIU Jia-mei, et al   

  1. Department of Histology and Embryology, School of Basic Medical Science, Jilin University, Changchun 130021, Jilin, China
  • Received:2003-12-12 Published:2004-01-25 Online:2004-01-25

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摘要： 目的检测神经组织细胞特异性抗原及神经营养因子-3(NT-3)、乙酰胆碱转移酶(ChAT)在大鼠羊膜上皮细胞中的表达。方法从孕12—14d的Wistar大鼠羊膜中分离羊膜上皮细胞,通过免疫细胞化学检测神经组织细胞特异性抗原(MAP-2、NSE、GFAP、Nestin、Musashi)及ChAT、NT-3在羊膜上皮细胞中的表达。结果羊膜上皮细胞表达神经干细胞、神经细胞、神经胶质细胞特异性抗原,并且在羊膜上皮细胞中有ChAT与NT-3的表达。结论羊膜上皮细胞与神经组织细胞具有一定的同源性;羊膜可望作为治疗神经系统疾病新的细胞来源。

关键词: 神经组织细胞, 羊膜上皮细胞, 免疫细胞化学染色, 大鼠

Abstract: ObjectiveTo detect specific antigens of neural cells in amniotic epithelial cells(AECs) in rats. MethodsAECs were dissociated and purified from the amnion of pregnancy 12—14 d rats. The expression of specific markers of neural stem cells (Nestin, Musashi) and differentiated cells (MAP-2,NSE,GFAP) and ChAT, NT-3 in the AECs were detected by immunocytochemistry. ResultsThe cultured AECs displayed positive immunoreactivity to MAP-2, NSE, GFAP, Nestin and Musashi. In addition, the cells also demonstrated immunoreactivity to ChAT and NT-3. ConclusionAECs are similar with neural cells and it may be useful as a sustained source to improve outcome of neural stem cells transplantation.

Key words: neural cells, amniotic epithelial cells(AECs), immunocytochemistry, rats