《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (12): 719-721.

• 基础研究 • 上一篇    下一篇

脊髓损伤后慢性中枢性疼痛与脊髓背角P物质关系研究

刘志芳1; 戴红1; 高秀来2; 肖忠新1; 景鹏2   

  1. 1.首都医科大学公共卫生与家庭医学院 北京市 100054;2.首都医科大学基础医学院 北京市 100054
  • 收稿日期:2003-11-10 出版日期:2003-12-25 发布日期:2003-12-25

Relationship of chronic central pain and substance P in spinal dorsal horn after spinal cord injury

LIU Zhi-fang, DAI Hong, GAO Xiu-lai, et al   

  1. School of Public Health and Family Medicine, Capital University of Medicine Sciences, Beijing 100054, China
  • Received:2003-11-10 Published:2003-12-25 Online:2003-12-25

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摘要： 目的探讨脊髓损伤(SCI)后慢性中枢性疼痛(CCP)与P物质的关系。方法选取SD大鼠28只,分为正常组(A组)、假手术组(B组),以及用WADE法复制出SCI后无CCP组(C组)和CCP组(D组 )。取大鼠T13和L2脊髓节段,采用免疫荧光组织化学染色法结合激光共聚焦显微镜技术观察脊髓背角P物质(SP)的变化。结果各组大鼠T13 和L2节段脊髓背角SP含量比较为:D组较C组减少(P<0.05),较A组和B组明显减少(P<0.01);C组较A组和B组减少(P<0.05);A组与B组无显著性差异。结论SCI后CCP大鼠脊髓背角SP可能对CCP有某种程度的抑制作用。

关键词: 脊髓损伤, 慢性中枢性疼痛, 脊髓背角, P物质, 免疫组织化学法, 共聚焦激光扫描显微镜

Abstract: Objective To approach the neurobiochemical mechanism of chronic central pain (CCP) after spinal cord injury (SCI). Methods 28 SD rats were divided into four groups, the normal group (group A), the pseudosurgery group (group B), and groups with CCP (group C) and without CCP (group D) after L1 spinal cord section injured with WADE method. T13 and L2 segments of rats' spinal cord were took and concentration changes of substance P (SP) in the spinal dorsal horn between two sections were examined by immunofluorescence histochemistry staining combined with confocal laser scanning microscope. Results Concentration of SP in the group D was decreased significantly compared with groups C,A and B (P<0.05-0.01), that of the group C was less than that of group A and B (P<0.05). Conclusion The rat model established by WADE method is proper to study CCP after SCI. SP in dorsal horn of spinal cord may inhibit the CCP after SCI in some degrees.

Key words: spinal cord injury (SCI), chronic central pain, spinal dorsal horn, substance P (SP), immunohistochemical assay, confocal laser scanning microscope