《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (11): 660-661.

• 基础研究 • 上一篇    下一篇

直流小电场诱导乳腺癌细胞凋亡及其机制的初步研究

李晓瑛1; 王兴林1; 赵坡2   

  1. 1.解放军总医院理疗科 北京市 100853;2.解放军总医院病理科 北京市 100853
  • 收稿日期:2003-08-18 出版日期:2003-11-25 发布日期:2003-11-25

Apoptosis of breast cancer cell line induced by small direct-current electric field and its mechanism

LI Xiao-ying, WANG Xing-lin,ZHAO Po   

  1. Department of Physiotherapy, The General Hospital of PLA, Beijing 100853, China
  • Received:2003-08-18 Published:2003-11-25 Online:2003-11-25

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被引次数

5

摘要： 目的研究直流小电场对于乳腺癌细胞凋亡的诱导作用及其可能机制。方法应用体外直流小电场作用于乳腺癌细胞系MCF7,观察其对乳腺癌细胞凋亡和重要基因P53、Rb以及E2F1表达的影响。 结果直流小电场对乳腺癌细胞有明显的诱导作用,200mV/mm组癌细胞大量脱落,电场组凋亡癌细胞数目随电场强度升高而增多(P<0.01)。电场组P53、Rb基因mRNA表达明显升高,E2F1基因mRNA表达明显下降。结论直流小电场对乳腺癌细胞具有明显的凋亡诱导作用并可能与P53、Rb以及E2F1基因的表达相关。

关键词: 乳腺肿瘤, 直流电场, 凋亡, 基因

Abstract: ObjectiveTo research the apoptosis of breast cancer cells by application of small direct-current electric field(EF) and its related possible mechanism.MethodsSmall direct-current EFs were applied in human breast cancer cell line MCF7 in vitro to observe the effects on apoptosis of cancer cells by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)and to detect the mRNA expression of p53, Rb and E2F1 genes by reverse transcriptase-polymerase chain reaction (RT-PCR).ResultsA lot of cancer cells were observed slaughed from culture dish by 200mV/mm of the field strength and the number of the apoptotic cancer cells was gradually significantly increased with the application of EFs(P<0.01). mRNA expression level for p53 and Rb genes was elevated but that for E2F1 gene was decreased overtly in cancer cells exposed to EFs.ConclusionApoptosis of breast cancer cells can be induced by small direct-current EFs and the change for expression of p53, Rb and E2F1 genes may be involved in the mechanism.

Key words: breast neoplasm, direct-current electric field, apoptosis, gene