《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (10): 631-632.

• 临床康复 • 上一篇    下一篇

肝硬化患者血清内毒素调节蛋白水平及其临床意义

刘静波1,2;张泰昌3   

  1. 1.首都医科大学康复医学院 北京市 100068;2.北京博爱医院内科 北京市 100068;3.首都医科大学宣武医院消化内科 北京市 100053
  • 收稿日期:2003-06-20 出版日期:2003-10-25 发布日期:2003-10-25

Change of lipopolysaccharide activity-modulating protein in serum of cirrhosis patients and its clinical significance

LIU Jing-bo,ZHANG Tai-chang   

  1. Department of Internal Medicine, Beijing Charity Hospital, Beijing 100068, China
  • Received:2003-06-20 Published:2003-10-25 Online:2003-10-25

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摘要： 目的探讨检测伴肠源性内毒素血症的肝硬化患者血中内毒素结合蛋白(LBP)、杀菌性/通透性增加蛋白(BPI)及可溶性sCD14水平的临床意义。方法应用基质显色法和ELISA双抗体夹心法,检测45例肝硬化患者血中内毒素脂多糖(LPS)、LBP、BPI和sCD14水平,并以15例健康献血员作为对照。结果肝功能为A级、B级和C级的肝硬化患者其血中LPS、LBP、BPI和sCD14水平均明显高于献血员;肝硬化死亡患者的LPS、LBP、BPI和sCD14水平显著高于存活者。结论肝硬化患者伴肠源性内毒素血症时,血清LBP和sCD14水平升高和BPI相对不足,可显著提高机体对LPS的敏感性。

关键词: 肝硬化, 内毒素, 内毒素结合蛋白, 杀菌性/通透性增加蛋白, 可溶性CD14

Abstract: ObjectiveTo explore changes of lipopolysaccharide (LPS), lipopolysaccharide-binding protein (LBP), bactericidal/permeability-increasing protein (BPI), soluble CD14 (sCD14) in blood of cirrhosis patients and their clinical significance.MethodsSerum samples of 45 cirrhosis patients were detected with chromogenic limulus amebocyte lysate assay for LPS and detected with ELISA for LBP, BPI and sCD14. While, serum samples of 15 normal subjects were used as controls.ResultsLevels of LPS, LBP, BPI and sCD14 in blood of cirrhosis patients with liver function being grade A, B and C were significantly higher than that in normal subjects. Also, those indexes fore mentioned were obviously higher in died cirrhosis patients than that in survived cirrhosis patients.Conclusion High levels of LBP, LPS and relative deficiency of BPI in cirrhosis patients accompanied with intestinal endotoxemia (IETM) may significantly increase the sensitivity of body to endotoxin.

Key words: cirrhosis, lipopolysaccharide (LBP), lipopolysaccharide-binding protein (BPI), bactericidal/permeability-increasing protein, soluble CD14 (sCD14)