《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (09): 533-535.

• 专题研究 • 上一篇    下一篇

中药SSY-B2对大鼠穹窿伞切断后神经元和神经生长因子表达的影响

陆华宝1,2; 李林1; 安文林1; 张丽1; 叶翠飞1   

  1. 1.首都医科大学宣武医院 北京市 100035;2.北京博爱医院 北京市 100068
  • 收稿日期:2003-03-17 出版日期:2003-09-25 发布日期:2003-09-25

Effect of SSY-B2 on loss of neurons and enhancing the expression of nerve growth factor of rats with bilateral fornix/fimbria transection

LU Hua-bao,LI Lin,AN Wen-lin,et al   

  1. Beijing Xuanwu Hospital,Beijing 100035,China
  • Received:2003-03-17 Published:2003-09-25 Online:2003-09-25

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被引次数

38

摘要： 目的评价中药制剂SSY-B2对神经元丧失及神经生长因子(NGF)表达的影响。方法成年雄性SD大鼠随机分为6组:假手术组、模型组、阳性对照药脑复康治疗组、SSY-B2小剂量治疗组(15g生药/kg),SSY-B2中剂量治疗组(3g生药/kg),SSY-B2大剂量治疗组(6g生药/kg)。采用切断大鼠双侧隔海马穹窿伞的模型,术后给予药物治疗或灌水6周,在大鼠行为学测试结束后,采用尼氏染色和免疫组织化学方法,对内侧隔区的神经元和不同脑区的NGF阳性神经元进行测量。结果模型组内侧隔区神经元数减少(20.33±14.01),SSY-B2小剂量组(59.13±22.02)、中剂量组(50.60±23.18)、大剂量组(63.93±18.35)与模型组相比,P<0.005。模型组内侧隔核、海马齿状回多形层和内嗅皮层/下托区的NGF阳性细胞与假手术组相比有所减少(P>0.05)。在内侧隔核,SSY-B2各剂量均能明显促进NGF的表达(均P<0.005)。在海马齿状回多形层和内嗅皮层/下托区,SSY-B2小、中剂量均能促进NGF的表达(P<0.05)。结论SSY-B2减少了大鼠内侧隔核神经元的丧失,这可能与此区NGF的表达增强有关;SSY-B2使海马齿状回、下托和内嗅皮层NGF表达增强,有助于这些区域发挥一定的学习记忆代偿功能。

关键词: 中药, SSY-B2, 神经元, 神经生长因子(NGF)

Abstract: ObjectiveTo evaluate the possible effect of SSY-B2 on reducing the loss of neurons and enhancing the expression of nerve growth factor(NGF).MethodsMale adult SD rats were divided randomly into 6 groups as sham group,model group,positive control agent piracetam group, SSY-B2 low(1.5g crude drug/kg), medium(3g crude drug/kg)and high dosage (6g crude drug/kg)group.Bilateral fornix/fimbria transection was carried out in the rats' septohippocampal pathway and 6 weeks' drug treatment was administered with different doses of SSY-B2 and positive control agent piracetam. After behavioral tests, the numbers of neurons in medial septum and immunoreactive products of NGF in different areas were measured, using Nissle staining and immunohistochemical methods.ResultsThere was neural loss in medial septum after fornix /fimbria transection, but SSY-B2 at each dosage markedly reduced the loss(59.13±22.02,50.60±23.18,63.93±18.35,the number of neurons for three SSY-B2 dosage groups,P<0.005 for all compared with the model group 20.33±14.01).The number of NGF positive cells decreased in model group, but did not show significant statistic difference compared with the sham group (P>0.05) in the medial septum, polymorph layer of dentate gyrus and entorhinal cortex/Subiculum area. In the medial septum, all three dosage enhanced the expression of NGF positive cells(145.1±57.7,161.3±08.2,200.6±58.2,the number of neurons for three SSY-B2 dosage group,P<0.005 for all compared with the model group 50.2±48.6). SSY-B2 at low and medium dosage group also increased the number in both entorhinal cortex/Subiculum and polymorph layer of dentate gyrus.Conclusions SSY-B2 can reduce the loss of neurons in the medial septum, which may be involve in increasing expression of NGF;NGF expression in dentate gyrus, subiculum of hippocampal formation and entorhinal cortex increased by SSY-B2 may play a role in the compensation of these area for learning/memory.

Key words: Traditional Chinese Medicine, SSY-B2, neurons, nerve growth factor(NGF)