《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (05): 282-284.

• 基础研究 • 上一篇    下一篇

脉冲磁针仪对脑缺血模型大鼠神经生长因子的影响

张海峰1; 唐强2   

  1. 1.浙江省中医院针灸科 浙江杭州市 310006;2.黑龙江中医药大学针灸推拿学院 黑龙江哈尔滨市 150001
  • 收稿日期:2003-03-05 出版日期:2003-05-25 发布日期:2003-05-25

Effect of Pulse Magnetic Needle Apparatus on nerve growth factor expression in cerebral ischemia model of rats

ZHANG Hai-feng,TANG Qiang   

  1. Department of Acupuncture and Moxibustion,Zhejiang Traditional Chinese Medicine Hospital,Hangzhou 310006,Zhejiang,China
  • Received:2003-03-05 Published:2003-05-25 Online:2003-05-25

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摘要： 目的研究脉冲磁针仪对脑缺血模型大鼠神经生长因子的影响。方法健康Wistar大鼠70只,雌雄各半,随机分为7组:正常组、假手术组、模型组、静磁组、针刺组、电针组、脉冲磁组。线栓法制备大鼠脑缺血模型,神经功能评分评定术后各组大鼠的神经功能,运用免疫组化方法检测各组神经生长因子变化情况。结果术后15d与术后6h组内神经功能评分比较 ,模型组无显著性差异(P>0.05),静磁组有显著性差异(P<0.05),其余各治疗组组内有高度显著性异差(P<0.01);组间15d时神经功能评分比较,脉冲磁组、电针组和针刺组疗效相仿。在第15d时神经生长因子表达比较,脉冲磁组、针刺组高于静磁组、电针组(P<0.05),静磁组、电针组高于模型组(P<0.05),针刺组与脉冲磁组无显著性差异(P>0.05)。结论脉冲磁针仪可促进神经生长因子产生并延长神经生长因子产生时限,促进脑缺血损伤后肢体功能恢复。

关键词: 脉冲磁针仪, 脑缺血, 神经生长因子

Abstract: ObjectiveTo study the effects of Pulse Magnetic Needle Apparatus on nerve growth factor(NGF) expression in cerebral ischemia model of rats.Methods70 Wistar rats were divided randomly into 7 groups as normal group,sham group,model group,magnetic group,acupuncture group,elecupuncture group and pulse magnetic group.Cerebral ischemia model was made by middle cerebral artery occlusion.The neural function of all the rats after operation was assessed by Nervous Scale of Function.The expression of NGF in each group was determined using immunohistochemical method.ResultsIn the Nervous Scale of Function between 6h and 15d after operation,there was no difference in model group(P>0.05),but there was difference in magnetic group(P<0.05) and in other therapeutic groups(P<0.01). The curative effect in pulse magnetic group was as the same as that in elecupuncture group and acupuncture group on the 15th day after operation.The expression of NGF increased in all therapeutic groups compared with the model group on the 15th day after operation(P<0.05 or 0.01).Furthermore, the expression of NGF increased in pulse magnetic group and acupuncture group rather than that in magnetic group and elecupuncture group(P<0.05).There was no distinct difference between acupuncture group and pulse magnetic group(P>0.05).ConclusionsThe Pulse Magnetic Needle Apparatus can promote and prolong the expression of NGF and recover the function of limbs.

Key words: Pulse Magnetic Needle Apparatus, cerebral ischemia, nerve growth factor