《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (02): 79-81.

• 基础研究 • 上一篇    下一篇

肿瘤细胞裂解物致敏树突状细胞对小鼠乳腺癌作用的研究

贾鑫; 李荣; 徐迎新; 夏绍友; 李力   

  1. 中国人民解放军总医院普通外科 北京市 100853
  • 收稿日期:2003-11-19 出版日期:2004-02-25 发布日期:2004-02-25

Effect of dendritic cells sensitized by tumor cells lysates on the murine breast cancer

JIA Xin, LI Rong, XU Ying-xin, et al   

  1. Department of General Surgery, General Hospital of PLA, Beijing 100853, China
  • Received:2003-11-19 Published:2004-02-25 Online:2004-02-25

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被引次数

21

摘要： 目的观察肿瘤细胞裂解物致敏树突状细胞(DC)对小鼠乳腺癌的治疗作用。方法无菌取小鼠骨髓细胞,在体外培养条件下经细胞因子作用诱导为树突状细胞(DC),用EMT6肿瘤抗原裂解物冲击致敏DC细胞,检测DC体外刺激活化淋巴细胞作用,以及经DC免疫产生的细胞毒T淋巴细胞(CTL)体外杀伤肿瘤细胞的活性,观察致敏DC免疫对小鼠乳腺癌模型的治疗作用。结果镜下可见抗原致敏后的DC可吸引淋巴细胞聚集成团;致敏DC诱导生成的特异性CTL在体外对肿瘤细胞可产生杀伤作用(与PBS对照组比较,P=0.027),而未成熟DC细胞组和肿瘤抗原组与PBS对照组间无显著性差异(P=0.17,P=0.072);经致敏DC注射免疫后,小鼠负荷肿瘤得到抑制(与PBS对照组比较,P=0.035),而单纯肿瘤抗原及未致敏DC免疫组与PBS对照组间无显著性差异(P=0.26,P=0.11)。结论肿瘤抗原裂解物致敏的DC可有效递呈抗原并诱导淋巴细胞杀伤肿瘤细胞。

关键词: 树突状细胞, 乳腺肿瘤, 免疫治疗

Abstract: ObjectiveTo investigate the effect of dendritic cells (DC) sensitized by tumor cells lysates on the murine breast cancer.MethodsBone marrow-generated DCs were sensitized by EMT6 tumor cell lysates, and tested whether they produced antigens to T lymphcytes and induced tumor-specific CTL. Antigen-sensitized DCs were also used to treat the mice with breast carcinoma.ResultsDCs sensitized by tumor cell lysates markedly led to the proliferation of lymphcytes in vitro, and induced tumor specific CTL that significantly difference compared with PBS control group (P=0.027). Meanwhile, DCs sensitized by tumor lysates could inhibit the growth of established murine breast cancer that also significantly difference compared with PBS control group (P=0.035).ConclusionDCs sensitized by tumor cell can induce an effective antitumor immune response both in vitro and in vivo.

Key words: dendritic cell, breast carcinoma, immunotherapy