《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (03): 189-190.

• 基础研究 • 上一篇    下一篇

抑肽酶和肿瘤坏死因子-α抗体预防术后腹腔粘连实验研究

杨雁灵1; 曹华梁2; 徐小平3; 岳树强1   

  1. 1.第四军医大学西京医院肝胆外科 陕西西安市 710032;2.西安市中心医院普通外科 陕西西安市 710003;3.第一军医大学珠江医院普通外科 广东广州市 510282
  • 收稿日期:2002-12-16 出版日期:2003-03-25 发布日期:2003-03-25

Preventive effct of Aprotinin and TNF-α antibody on postoperative abdominal adhesion in mice

YANG Yan-ling,CAO Hua-liang,XU Xiao-ping,et al   

  1. Department of Hepatobiliary Surgery,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shanxi,China
  • Received:2002-12-16 Published:2003-03-25 Online:2003-03-25

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被引次数

29

摘要： 目的研究抑肽酶和肿瘤坏死因子-α(TNF-α)抗体预防小鼠术后腹腔粘连的疗效。方法 96只BALB/c小鼠随机分成4组:生理盐水对照组、抑肽酶组、TNF-α抗体组、抑肽酶联合TNF-α抗体组,每组24只。经过同一标准的腹膜损伤手术后,分别在关腹前腹腔内灌注生理盐水及抑肽酶和相应抗体。术后20d将小鼠处死,观察腹腔内粘连的情况,通过对粘连的程度、位置以及粘连类型的综合评价得出粘连分数。结果抑肽酶联合TNF-α抗体组的平均粘连分数和2—3级粘连发生率明显低于其他各组(P<0.01)。结论抑肽酶和TNF- α抗体能够对术后腹腔粘连形成起预防作用。

关键词: 抑肽酶, 肿瘤坏死因子α, 腹膜粘连, 小鼠

Abstract: ObjectiveTo study the effect of aprotinin and tumor necrosis factor alpha(TNF-α) antibody on preventing postoperative abdominal adhesion in mice.Methods96 BALB/c mice were randomly divided into 4 groups:normal saline group(n=24),aprotinin group(n=24),TNF-α antibody group(n=24),and aprotinin combined with TNF-α antibody group(n=24).After an identical peritoneal injury operation,all mice were treated intraperitoneally with normal saline , aprotinin,TNF-α antibody and aprotinin combined with TNF-α respectively at the time of abdominal closure .The mice were killed 20 days after operation,the abdominal incisions and the development of intraabdominal adhesions were observed.Adhesion score was based on an overall assessment of the extent,location,and type of adhesions .ResultsAprotinin combined with TNF-α antibody group showed significantly lower adhesion score and 2-3 grade adhesion(advanced significant adhesion) rate was lower than that of other groups(P<0.01).Conclusions The intraperitoneal administration of aprotinin and TNF-α antibody can inhibit postoperative adhesion formation.

Key words: peritoneal adhesion, Aprotinin, tumor necrosis factor α, mice