《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (12): 1391-1396.

• 国际会议报道 • 上一篇    下一篇

骨髓间充质干细胞静脉移植对脊髓损伤大鼠生长相关蛋白-43表达的影响

李兵奎 1，曾彬 2，常巍 1，王达义 1，杨棋 1
  

  1. 1.湖北省十堰市太和医院(湖北医药学院附属医院)脊柱外科，湖北十堰市 442000；2.湖北医药学院附属东风公司总医院内科，湖北十堰市 442000。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-12-25 发布日期:2015-12-25

Effects of Bone Marrow Mesenchymal Stem Cells Transplantation on Expression of Growth Associated Protein-43 in Rats after Spinal Cord Injury

LI Bing-kui1, ZENG Bin2, CHANG Wei1, WANG Da-yi1, YANG Qi1
  

  1. 1. Department of Spinal Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China; 2. Department of Internal Medicine, Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-12-25 Online:2015-12-25

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摘要： 目的 观察骨髓间充质干细胞(BMSCs)静脉移植对脊髓损伤(SCI)大鼠生长相关蛋白(GAP)-43 基因及蛋白表达的影响，探讨BMSCs静脉移植治疗SCI的作用机制。方法 采用SCI打击器建立大鼠T11脊髓撞击损伤模型，造模成功后，将大鼠随机分为对照组(n=18)和观察组(n=18)。SCI后 1周，对照组大鼠自尾静脉注射无血清的 DMEM/F12培养液 0.1 ml，观察组大鼠自尾静脉注射BMSCs悬液0.1 ml。SCI后1、2、4、8周，两组大鼠均进行BBB后肢运动功能评分；SCI后2、4、8周，采用RT-PCR检测GAP-43 mRNA的表达，采用免疫组化染色检测 GAP-43蛋白的表达。结果 与对照组比较，观察组 SCI后 2、4、8周 BBB评分显著升高(P<0.001)，GAP-43 阳性表达显著增强(P<0.001)，GAP-43 mRNA 的相对表达量升高(P<0.05)。结论 BMSCs 移植能增强SCI大鼠GAP-43在基因及蛋白水平的表达，从而促进SCI的再生修复。

关键词: 脊髓损伤, 骨髓间充质干细胞, 移植, 生长相关蛋白-43, 大鼠

Abstract: Objective To observe the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the expression of growth associated protein-43 (GAP-43) in rats after spinal cord injury (SCI). Methods The SCI models were made by impacting at the level of T11 segment. After modeling, the rats were randomly and equally divided into control group (n=18) and observation group (n=18). 1 week after injury, DMEM/F12 medium 0.1 ml was injected into tail vein of rats in the control group, and an equal volume of BMSCs suspension was injected in the observation group. 1, 2, 4, 8 weeks after SCI, the hindlimb function was evaluated by the Basso-Beattie-Bresnahan (BBB) score. 2, 4, 8 weeks after SCI, the expression of GAP-43 mRNA was detected by RT-PCR, and the expression of GAP-43 was detected by the immunohistochemistry. Results 2, 4, 8 weeks after SCI, compared with the control group, The BBB score increased, the expression of GAP-43 mRNA and GAP-43 increased in the observation group (P<0.05). Conclusion BMSCs transplantation can improve the expression of GAP-43 in gene and protein level, and then promote the repair of SCI.

Key words: spinal cord injury, bone marrow mesenchymal stem cells, transplantation, growth associated protein-43, rats