《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (10): 1151-1155.

• 基础研究 • 上一篇    下一篇

针康法对局灶性脑缺血大鼠神经功能缺损及缺血区碱性成纤维细胞生长因子、血管抑制素蛋白表达的影响

唐强 1，姜云飞 2，朱路文 1，叶涛 2，宋名杨 2   

  1. 1.黑龙江中医药大学附属第二医院，黑龙江哈尔滨市 150001；2.黑龙江中医药大学，黑龙江哈尔滨市 150040。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-10-25 发布日期:2015-10-25

Effect of Cluster Needling of Scalp Acupuncture Combined with Rehabilitation on Neurological Function and Expression of Basic Fibroblast Growth Factor and Angiostatin in Rats after Focal Cerebral Ischemia

TANG Qiang1, JIANG Yun-fei2, ZHU Lu-wen1, YE Tao2, SONG Ming-yang2   

  1. 1. Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China; 2. Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-10-25 Online:2015-10-25

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摘要： 目的 探讨针康法对局灶性脑缺血大鼠神经功能缺损及缺血区碱性成纤维细胞生长因子(bFGF)和血管抑制素(AS)蛋白表达的影响。方法 采用随机数字表法将90只清洁健康级雄性Sprague-Dawley大鼠随机分为假手术组、模型组、针刺治疗组、康复治疗组和针康治疗组，各组又分为3 d、7 d、14 d 3个亚组(n=6)。改良Longa法制备永久性脑缺血大鼠模型。模型组与假手术组不予任何治疗，针刺治疗组采用头穴丛刺疗法，康复治疗组采用跑台训练，针康治疗组采用针康法治疗。术后3 d、7 d、14 d采用改良神经功能缺损评分(mNSS)评估各实验组大鼠的神经功能，Western blotting法检测各实验组大鼠缺血半暗区bFGF和AS蛋白的表达。结果 术后3 d、7 d、14 d，与模型组、针刺治疗组、康复治疗组比较，针康治疗组mNSS评分降低(P<0.05)，bFGF蛋白表达升高(P<0.05)， AS蛋白表达降低(P<0.05)。结论 针康法能降低局灶性脑缺血大鼠神经功能缺损，其机制可能与上调bFGF蛋白表达、下调AS蛋白表达有关。

关键词: 脑缺血, 神经功能, 针康法, 碱性成纤维细胞生长因子, 血管抑制素, 大鼠

Abstract: Objective To study the effect of cluster needling of scalp acupuncture combined with rehabilitation on neurological function and expression of basic fibroblast growth factor (bFGF) and angiostatin (AS) in rats after focal cerebral ischemia. Methods 90 male Sprague-Dawley rats were randomly divided into sham operation group, model group, acupuncture treatment group, rehabilitation treatment group and acupuncture and rehabilitation treatment group, each group was further divided into 3 days, 7 days and 14 days subgroups (n=6). Permanent cerebral ischemia rat model was established according to Longa's method. The model group and the sham operation group accepted no treatment, the acupuncture treatment group was treated by cluster needling of scalp acupuncture, the rehabilitation treatment group received the treadmill training, the acupuncture and rehabilitation treatment group was treated by acupuncture and rehabilitation therapy. 3 days, 7 days, 14 days after operation, the neural function was evaluated with the modified Neurological Severity Score (mNSS), the expression levels of bFGF and AS proteins were detected by Western blotting. Results Compared with the model group, the acupuncture treatment group and the rehabilitation treatment group, mNSS decreased, the expression of bFGF protein increased, and the expression of AS protein decreased in the acupuncture and rehabilitation treatment group 3 days, 7 days, 14 days after operation (P<0.05). Conclusion Acupuncture and rehabilitation therapy can reduce the neurological function defect in rats with focal cerebral ischemia, which may be related to the upregulation of bFGF protein expression and down-regulation of AS protein expression.

Key words: cerebral ischemia, neurological function, acupuncture and rehabilitation therapy, basic fibroblast growth factor, angiostatin, rats