《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (09): 1016-1019.

• 基础研究 • 上一篇    下一篇

音乐干预对孕期应激仔鼠恐惧行为的影响及相关机制

钱静 1，宓秀菊 2，刘树青 3   

  1. 1.北京儿童医院集团，聊城市儿童医院儿童保健科，山东聊城市 252000；2.聊城市人民医院儿科，山东聊城市 252000；3.北京儿童医院集团，贵阳市儿童医院保健部，贵州贵阳市 550003。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-09-25 发布日期:2015-09-25

Effect and Mechanism of Music Exposure on Fearful Behavior of Prenatal Stressed Rats

QIAN Jing1, MI Xiu-ju2, LIU Shu-qing3   

  1. 1. Department of Child Care, Beijing Children's Hospital Medical Group, Liaocheng Children's Hospital， Liaocheng, Shandong 252000, China; 2. Department of Pediatrics, Liaocheng People's Hospital, Liaocheng, Shandong 252000, China; 3. Department of Health Care, Beijing Children's Hospital Medical Group, Guiyang Children's Hospital, Guiyang, Guizhou 550003, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-09-25 Online:2015-09-25

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摘要： 目的 研究音乐干预对孕期应激仔鼠恐惧行为和杏仁核胃泌素释放肽受体(GRPR)表达的影响。方法 Wistar孕鼠 16只随机分为应激组(n=8)和对照组(n=8)。应激组妊娠 11~20 d每天将其置于窄小的固定器中并给予强光照射。对照组不给予刺激。应激组仔鼠出生后第1天起，予播放音乐(SM组)或相同分贝的白噪音(SW组)，SC组不给予刺激。生后60 d行防御退缩试验，免疫荧光组织化学和荧光实时定量 PCR测定杏仁核 GRPR及其 mRNA表达。结果 与其他两组比较，SM组仔鼠潜伏期显著缩短(P<0.001)，出小室次数显著增多(P<0.001)；杏仁核 GRPR阳性细胞计数及 mRNA表达显著增加(P<0.001)。结论 生后给予音乐干预可以纠正孕期应激仔鼠异常的恐惧行为，同时伴有杏仁核GRPR高表达。

关键词: 孕期应激, 音乐疗法, 胃泌素释放肽受体, 杏仁核, 防御退缩试验, 大鼠

Abstract: Objective To investigate the effect of postnatal music exposure on fearful behavior of prenatal stressed rats in defensive withdrawal test and the expression of gastrin-releasing peptide receptor (GRPR) in the amygdala. Methods Wistar pregnant rats were divided in stress group (n=8) and control group (n=8). The stress group were immobilized and bright lighted 45 min, 3 times a day, when gestation 11-20 days, while the control group without any stress. The new born rats from the stress group were exposed in music (SM group) or white noise (SW group) for 45 min twice a day since 1 day after born, and the rats from the control (SC) group left undisturbed. They were tested with defensive withdrawal test 60 days after birth, and the GRPR levels in the amygdala were measured with immunofluorescence and real-time PCR. Results The latency was shorter and the frequence out of the chamber was the most in the SM group of the defensive withdrawal test (P<0.001), while GRPR-positive cells and GRPR mRNA increased in amygdala (P<0.001). Conclusion Postnatal music exposure can counteract the abnormal behavior response of prenatal stressed offspring and accompany with increased expression of GRPR in the amygdala.

Key words: prenatal stress, music exposure, gastrin-releasing peptide receptor, amygdala, defensive withdrawal test, rats