《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (08): 894-899.

• 特稿 • 上一篇    下一篇

活血化瘀法对急性心肌梗死大鼠CD34、血管内皮生长因子阳性细胞的影响

师伟1，李晔紫2，赵海滨2，杨丹丹2，姜天元2，李东方2，翟瑶瑶2   

  1. 1.北京市朝阳区卫生局，北京市100026；2.北京中医药大学第三附属医院心内科，北京市100029。作者简介：师伟(1960-)，男，汉族，河北东光县人，博士，主任医师，主要研究方向：疾病预防控制工作。通讯作者：赵海滨，男，博士，教授。E-mail: haibin999@126.com。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-08-25 发布日期:2015-08-25

Effect of Activating Blood to Resolve Stagnation on Cells Expressing CD34 and Vascular Endothelial Growth Factor in Rats with Acute Myocardial Infarction

SHI Wei1, LI Ye-zi2, ZHAO Hai-bin2, YANG Dan-dan2, JIANG Tian-yuan2, LI Dong-fang2, ZHAI Yao-yao2   

  1. 1.Health Bureau of Chaoyang District, Beijing 100026, China; 2.Department of Cardiology, the Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-08-25 Online:2015-08-25

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摘要： 目的研究活血化瘀法对大鼠心肌组织微环境中CD34、血管内皮生长因子(VEGF)的影响，探讨其治疗急性心肌梗死的机制。方法Sprague-Dawley 大鼠32 只随机分为假手术组(n=8)、模型组(n=8)、中药+粒细胞集落刺激因子(G-CSF)组(n=8)和G-CSF组(n=8)。各组均于造模3 h 后给予相应药物，连续6 d，第7 天处死取材。苏木素-伊红(HE)染色观察病理形态，免疫组化染色检测梗死心肌边缘区CD34、VEGF、Ki-67 阳性细胞表达。结果模型组、中药+G-CSF组和G-CSF组CD34、VEGF、Ki-67 阳性细胞表达均高于假手术组(P<0.05)；中药+G-CSF 组、G-CSF 组高于模型组(P<0.05)；中药+G-CSF 组CD34、VEGF 阳性表达高于G-CSF组(P<0.05)，两组Ki-67 阳性细胞表达无显著性差异(P>0.05)。结论活血化瘀法能够提高CD34和VEGF的表达，其作用优于单独使用G-CSF，可以有效治疗急性心肌梗死。

关键词: 急性心肌梗死, 骨髓干细胞, 血管内皮生长因子, 微环境, 活血化瘀法

Abstract: Objective To explore the effect of Activating Blood to Resolve Stagnation on the expression of CD34 and vascular endothelial growth factor (VEGF) in rats with acute myocardial infarction. Methods 32 Sprague-Dawley rats were randomly divided into sham operation group (A, n=8), model group (B, n=8), Xuesaitong Injection + granulocyte colony- stimulating factor (G- CSF) group (C, n=8) and G-CSF group (D, n=8). Corresponding medicine was given to each group 3 hours after modeling, for 6 days. Pathomorphological changes were observed through HE staining, and the expression of CD34, VEGF and Ki-67 were observed through immunohistochemical staining. Results The expressions of CD34, VEGF and Ki-67 were higher in groups B, C and D than in group A (P<0.05), and were higher in group groups C and D than in group B (P<0.05). The expressions of CD34 and VEGF were higher in group C than in group D (P<0.05). However, there was no significant difference in the expression of Ki-67 between 2 groups (P>0.05). Conclusion The expression of CD34 and VEGF increases with Activating Blood to Resolve Stagnation method, which is superior to using G-CSF only. Activating Blood to Resolve Stagnation may play an important role in the treatment of acute myocardial infarction.

Key words: acute myocardial infarction, marrow stem cells, vascular endothelial growth factor, micro environment, Activating Blood to Resolve Stagnation method