《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (04): 412-416.

• 基础研究 • 上一篇    下一篇

丁苯酞对SH-SY5Y细胞凋亡的影响

赵素晨 1，吴庆文 1，程月发 2，陈娟 1，贾玉凤 1，孟奇 3
   

  1. 1.河北联合大学护理与康复学院，河北唐山市 063000；2.河北联合大学冀唐学院，河北唐山市 063000；3.四川大学华西公共卫生学院，四川成都市 610041。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-04-25 发布日期:2015-04-25

Effect of Butylphthalide on Apoptosis of SH-SY5Y Cells

ZHAO Su-chen1, WU Qing-wen1, CHENG Yue-fa2, CHEN Juan1, JIA Yu-feng1, MENG Qi3
   

  1. 1. College of Nursing and Rehabilitation of Hebei United University, Tangshan, Hebei 063000, China; 2. Jitang College of Hebei United University, Tangshan, Hebei 063000, China; 3. West China School of Public Health, Sichuan University, Chengdu, Sichuan 610041, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-04-25 Online:2015-04-25

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摘要： 目的 探讨丁苯酞对 l-甲基-4-苯基-吡啶离子(MPP+)诱导的 SH-SY5Y细胞凋亡的影响。方法 SH-SY5Y细胞加入 1 µmol/L、10 µmol/L、20 µmol/L丁苯酞培养2 h，加入MPP+培养24 h。台盼蓝染色检测SH-SY5Y细胞存活率；罗丹明123染色检测 线粒体膜电位的变化；ELISA检测胞浆 P53的含量；Western blotting检测胞浆细胞色素 C (CytC)、P53的表达。结果 随丁苯酞剂 量提高，SH-SY5Y细胞存活率提高(P<0.05)，线粒体膜电位降低(P<0.05)，P53和 CytC蛋白表达下降(P<0.05)。结论 丁苯酞能够 抵抗MPP+诱导的SH-SY5Y细胞凋亡，其机制可能是通过抑制P53、CytC的表达，增加细胞线粒体膜稳定性。

关键词: 帕金森病, 丁苯酞, SH-SY5Y细胞, P53, 细胞色素C, 凋亡

Abstract: Objective To explore the effects of butylphthalide on the apoptosis of SH-SY5Y cells induced by the 1-methyl-4-phenylpyridinium iodide (MPP+). Methods SH-SY5Y cells were cultured with butylphthalide in the dosage of 1 µmol/L, 10 µmol/L and 20 µmol/L for 2 hours, and with MPP+ for 24 hours. The viability of cells was measured with trypan blue. The mitochondrial membrane potential was detected with Rhodamine 123. The content of P53 protein was detected with ELISA. The expression of P53 and cytochrome C (CytC) were detected with Western blotting. Results The viability of SH-SY5Y cells increased with the dosage of butylphthalide (P<0.05), while the mitochondrial membrane potential and the expression of P53 and CytC decreased (P<0.05). Conclusion Butylphthalide can prevents the SH-SY5Y cells from the injury induced by MPP+, which may associate with the inhibition of apoptosis through the expression of P53 and CytC.

Key words: Parkinson's disease, butylphthalide, SH-SY5Y cells, P53, cytochrome C, apoptosis