《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (05): 514-518.

• 特稿 • 上一篇    下一篇

时钟基因启动子甲基化频率随增龄的组织特异性改变

祝艳秋1a，陆璐1a，李林1a，蔡彦宁1b，张兰1a   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-05-25 发布日期:2015-05-25

Tissue-specific Changes of Clock DNA Promoter Methylation with Aging

ZHU Yan-qiu1a, LU Lu1a, LI Lin1a, CAI Yan-ning1b, ZHANG Lan1a   

  1. 1. a. Department of Pharmacology, b. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-05-25 Online:2015-05-25

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摘要： 目的探索时钟基因启动子甲基化频率增龄性改变及其在衰老中的作用。方法4 月龄(青年组，n=9)、20 月龄(老年组，n=10) C57BL小鼠，采用甲基化特异性聚合酶链反应方法检测小鼠胃、脾、血管、肾、纹状体中时钟基因Per1/2、Bmal1/2、Cry1/2、Clock、Npas2 启动子甲基化水平。结果老年组脾组织Cry1、Bmal2 和NPas2 启动子区甲基化频率高于青年组(P<0.05)，胃组织Per1 基因启动子甲基化频率低于青年组(P<0.05)，血管组织Bmal1 启动子区甲基化频率高于青年组(P<0.05)。结论多种时钟基因启动子甲基化可能参与衰老进程，并具有组织特异性。

关键词: 衰老, 昼夜节律, 时钟基因, 启动子甲基化

Abstract: Objective To investigate the role of the clock gene promoter methylation in aging. Methods C57BL mice of 4- (young, n=9) and 20- (old, n=10) month-old were determined the promoter methylation level of clock genes (Per1/2, Bmal1/2, Cry1/2, Clock, Npas2) in the stomach, spleen, vascular, kidney and striatum with methylation-specific polymerase chain reaction (MSP). Results The incidence of promoter methylation of Cry1, Bmal2 and Npas2 in spleen increased in old mice (P<0.05), while the promoter methylation of Per1 in stomach decreased (P<0.05), and the promoter methylation of Bmal1 in vascular increased (P<0.05). Conclusion Promoter methylation of some clock genes is involved in process of aging in a tissue-specific way.

Key words: aging, circadian rhythm, clock genes, promoter methylation