《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (02): 142-147.

• 基础研究 • 上一篇    下一篇

大鼠胸髓横断后腰髓下运动神经元的变化

熊国星1，洪毅2,3，张军卫2,3，陈世铮2,3，关骅2,3   

  1. 1.首都医科大学公共卫生学院康复医学教研室，北京市100069；2.首都医科大学康复医学院，北京市100068；3.中国康复研究中心北京博爱医院脊柱脊髓外科，北京市100068。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-02-25 发布日期:2015-02-25

Functional and Structural Changes of Lower Motor Neuron Distal to the Site of Rats with Spinal Cord Transection at T10

XIONG Guo-xing, HONG Yi, ZHANG Jun-wei, CHEN Shi-zheng, GUAN Hua   

  1. Department of Rehabilitation, School of Public Health, Capital Medical University, Beijing 100069, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-02-25 Online:2015-02-25

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摘要： 目的探讨脊髓损伤后远端下运动神经元结构、功能的改变。方法将70 只Sprague-Dawley 大鼠随机分为T10脊髓横断3 d 组(n=10)、1 周组(n=10)、2 周组(n=10)、4 周组(n=15)、8 周组(n=15)和假手术组(n=10)，进行L4-6脊髓节段TUNEL荧光标记、AChE酶组化定量检测。结果TUNEL标记法观察损伤远端脊髓前角的细胞凋亡情况，结果仅偶见荧光标记物。脊髓横断3 d 时，AChE 阳性运动神经元(面积>300 μm2)的OD值明显减小(P<0.01)；损伤1 周时，OD值突然明显增大(P<0.01)；随后OD值再次下降，至4 周时降至最低点，然后再次增加，8 周时与假手术组仍存在显著性差异(P<0.05)。结论脊髓损伤后远端下运动神经元没有明显减少及结构的不可逆改变，但代谢功能发生显著的可逆性变化。

关键词: 脊髓损伤, 跨神经元变性, 功能重组

Abstract: Objective To investigate the structural and functional changes of lower motor neuron distal to the site of spinal cord injury in rats. Methods Seventies Sprague-Dawley rats were divided randomly into 6 groups: sham-operation group (controls, n=10) and 3 day group (n=10), 1 week group (n=10), 2 week group (n=10), 4 week group (n=15) and 8 week group (n=15) after spinal cord transaction at T10. Neuronal apoptosis and acetylcholinesterase (AChE) activity of spinal cord at L4- 6 were observed by using the terminal deoxynucleotidal transferase- mediated DUTP-biotin nick end labeling (TUNEL) method and the semiquantitative enzyme cytochemistry, respectively. Results The assessment of apoptosis by TUNEL labeling showed that fluorescent markers were observed occasionally in anterior horn distal to the site of injury. The optical density (OD) value of AchE positive motor neurons (area > 300 μm2) initially decreased about 3 days after transaction and then overshot 1 week or so. However, after that, the OD value decreased again, the lowest about 4 weeks. Then the OD value increased again, though at 8 weeks was still lower than that of controls (P<0.05). Conclusion The findings on indistinctive apoptosis provided the proof of no significant changes of lower motor neuron distal to the site of transection. Semiquantitative histochemical results about AChE reflected marked metabolic changes of motoneurons caudal to the transaction, which represented as part of functional reorganization.

Key words: spinal cord injury, transneuronal degeneration, functional reorganization