《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (01): 35-038.

• 基础研究 • 上一篇    下一篇

电针对局灶性脑缺血大鼠10 号染色体同源丢失性磷酸酶张力蛋白和神经生长相关蛋白-43 表达的影响

张鹏飞1，徐成成2，李文磊2，郭海英1
   

  1. 1.南京中医药大学，江苏南京市210029；2.南京中医药大学附属医院江苏省中医院神经内科，江苏南京市210029。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-01-25 发布日期:2015-01-25

Effects of Electroacupuncture on Expressions of Phosphatase and Tensin homology Deleted on Chromosome Ten and Growth Associated Protein 43 in Rats with Barrel Cortex Ischemia

ZHANG Peng-fei, XU Cheng-cheng, LI Wen-lei, GUO Hai-ying.   

  1. Nanjing University of Traditional Chinses Medicine, Nanjing, Jiangsu 210029, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-01-25 Online:2015-01-25

PDF (PC)

被引次数

7

摘要： 目的探讨电针对胡须体觉皮层局灶性脑缺血大鼠10 号染色体同源丢失性磷酸酶张力蛋白(PTEN)和神经生长相关蛋白43(GAP-43)表达的影响。方法24 只成年健康雄性Sprague-Dawley 大鼠分为假手术组(n=8)、模型组(n=8)及电针组(n=8)。于造模成功后第3 天，电针组予电针百会(DU20)和风府(DU16) 30 min，每天1 次。造模前1 d，给药后3 d、7 d 和14 d，应用角落测试进行行为学检测；给药后14 d 采用Western blotting 检测梗死周边区PTEN、GAP-43 蛋白的表达。结果电针组向右转的次数较模型组显著下降(P<0.001)。模型组GAP-43 表达较假手术组升高(P<0.05)，电针组较模型组升高(P<0.05)。模型组PTEN表达与假手术组无显著性差异(P=0.460)，电针组较模型组显著降低(P<0.001)。结论电针可能通过抑制PTEN和增加GAP-43 的表达促进脑缺血后神经再生和功能恢复。

关键词: 脑缺血, 电针, 神经再生, 10 号染色体同源丢失性磷酸酶张力蛋白, 生长相关蛋白-43, 大鼠

Abstract: Objective To investigate the effects of electroacupuncture (EA) on expression of phosphatase and tensin homology deleted on chromosome ten (PTEN) protein and growth associated protein-43 (GAP-43) in rats with Barrel cortex focal ischemia. Methods 24 adult male Sprague-Dawley rats were divided into sham group (n=8), model group (n=8) and EA group (n=8). The EA group accepted EA at Baihui (DU20) and Fengfu (DU16) acupoints 3 days after modeling, 30 min a time, once a day. They were assessed with Corner Test 1 day before modeling, and 3 days, 7 days and 14 days after medication. The expression of GAP-43 and PTEN around infarct zone were detect with Western blotting 14 days after medication. Results The frequence of turn-right decreased in the EA group compared with that in the model group (P<0.001) 14 days after modeling. The expression of GAP-43 increased in the model group compared with that in the sham group (P<0.05), and increased more in the EA group than in the model group (P<0.05). There was no significantly difference in PTEN expression between the model group and the sham group (P=0.460), and significantly decreased in the EA group (P<0.001). Conclusion EA may inhibit expression of PTEN protein and increase expression of GAP-43, which may be involved in nerve regeneration and functional recovery.

Key words: cerebral ischemia, electroacupuncture, nerve regeneration, phosphatase and tensinhomology deleted on chromosome ten, growth associated protein 43, rats