《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (01): 31-034.

• 专题 • 上一篇    下一篇

局灶性脑缺血再灌注大鼠神经血管单元的空间构筑

金媛媛1a，胡静1a，冯璐1a，李杨1a，陈烨鋆1a，胡晓松1b，李帅1b
   

  1. 1.成都医学院，a.临床医学系；b.形态学实验室， 四川成都市610500。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-01-25 发布日期:2015-01-25

Spatial Organization of Neurovascular Unit after Focal Cerebral Ischemia-reperfusion in Rats

JIN Yuan-yuan, HU Jing, FENG Lu,LI Yang, CHEN Ye-yun, HU Xiao-song, LI Shuai.
  

  1. Chengdu Medical College, Chengdu, Sichuan 610500, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-01-25 Online:2015-01-25

PDF (PC)

被引次数

4

摘要： 目的研究局灶性脑缺血不同再灌注时间段大鼠脑血管、神经元和星形胶质细胞的空间构筑。方法24 只Sprague-Dawley 大鼠随机分为假手术组(n=8)和模型组(n=16)，模型组再平均分为再灌注1 d 和2 周两个亚组。线栓法大鼠大脑中动脉闭塞1 h 后再灌注。各组活体灌注明胶墨汁。按时间取脑组织冰冻连续切片，免疫组织化学法观察胶质纤维酸性蛋白(GFAP)、神经元核心抗原(NeuN) 的表达。结果血管在皮质或神经核等处分布较多，多数星形胶质细胞突起与血管及神经元接触。模型组缺血侧GFAP表达较假手术组持续增多，NeuN表达减少。再灌注1 d 缺血侧血管数目明显减少，再灌注2 周后增多，但仍低于假手术组及非缺血侧。结论观察到脑缺血再灌注大鼠神经血管单元的构筑。

关键词: 局灶性脑缺血, 再灌注, 神经血管单元, 空间构筑, 大鼠

Abstract: Objective To investigate the spatial organization of neurons, blood vessel and astrocytes at different time of reperfusion after focal cerebral ischemia in rats. Methods 24 Sprague-Dawley rats were randomly divided into sham group (n=8), reperfusion 1 day group and reperfusion 2 weeks group. The latter 2 groups were occluded the middle cerebral arteries for an hour and reperfused. All the rats were injected with gelatin ink. The expressions of glial fibrillary acid protein (GFAP) and neuronal nuclear antigen (NeuN) in the brain were observed with immunohistochemistry. Results The vessels located mainly in cortex and nucleus. Most of astrocytes apophysis connected with vessels and neurons. Compared to the sham group, the expression of GFAP increased significantly in ischemic side, and the expression of NeuN decreased 1 day and 2 weeks after ischemia-reperfusion. The vessels decreased in the ischemic side 1 day after cerebral ischemia-reperfusion, and then increased 2 weeks later. Conclusion The organization of neurovascular unit after cerebral ischemia-reperfusion has been observed.

Key words: focal cerebral ischemia, reperfusion, neurovascular unit, spatial organization, rats