《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (01): 42-044.

• 础基研究 • 上一篇    下一篇

探索学习环境对脑梗死大鼠学习记忆能力及缺血侧海马区微血管密度的影响

马向阳，张丽萍，董静，黄冬冬，刘冬梅，孙汝智   

  1. 沧州医学高等专科学校，河北沧州市061001。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-01-25 发布日期:2015-01-25

Effects of Learning Environment on Learning and Memory and Microvessel Density in Hippocampus of Rats after Cerebral Infarction

MA Xiang-yang, ZHANG Li-ping, DONG Jing, HUANG Dong-dong, LIU Dong-mei, SUN Ru-zhi.
  

  1. Cangzhou Medical College, Cangzhou,Hebei 061001, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-01-25 Online:2015-01-25

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被引次数

1

摘要： 目的研究探索学习环境对局灶性脑梗死大鼠学习记忆能力及缺血侧海马区微血管密度的影响。方法80 只Sprague-Dawley 大鼠开颅电凝右侧大脑中动脉，随机分为标准环境组(SE 组, n=40)和探索学习环境组(LE 组, n=40)。分别于术后7d、28 d 进行Morris 水迷宫测试；术后1 d、3 d、7 d、14 d、28 d，CD34免疫组织化学法测定海马区微血管密度。结果LE组Morris水迷宫测试成绩显著优于SE组(P<0.001)。术后7 d 起，LE组缺血侧海马区微血管密度高于SE组(P<0.05)。结论探索学习环境可促进脑梗死大鼠微血管新生，有利于学习记忆功能恢复。

关键词: 脑梗死, 探索学习环境, 学习记忆, 微血管密度, 大鼠

Abstract: Objective To study the effects of learning environment on learning memory and microvessel density in hippocampus of rats after cerebral infarction. Methods 80 Sprague-Dawley rats were coagulated right middle cerebral arteries electrically, and randomly divided into learning envionment (LE) and standard environment (SE) groups. They were tested with Morris Water Maze 7 d and 28 d after operation. The microvessel density in hippocampus was measured with CD34 immunohistochemstry 1, 3, 7, 14 and 28 days after operation. Results The achievement of Morris Water Maze was significantly better in the LE group than in the SE group (P<0.001), while the microvessel density in the hippocampus was more since 7 days after operation (P<0.05). Conclusion The learning environment can promote the angiogenesis and improve the learning and memory function recovery.

Key words: cerebral infarction, learning environment, learning and memory, microvessel density, rats