《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (8): 709-712.

• 专题 • 上一篇    下一篇

N-叔丁基-α-苯基硝酮对大鼠脊髓损伤后神经生长因子表达的影响①

刘少辉，刘伟，邱少汕，徐锡金   

  1. 汕头大学医学院细胞生物学与遗传学教研室，广东汕头市515041
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-08-25 发布日期:2014-08-25

Effect of α-phenyl-N-tert-butylinitrone on Expression of Nerve Growth Factor in Spinal Cord Injured Rats

LIU Shao-hui, LIU Wei,QIU Shao-shan, XU Xi-jin   

  1. Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong 515041,China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-08-25 Online:2014-08-25

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摘要： 目的观察N-叔丁基-α-苯基硝酮(PBN)对脊髓损伤大鼠脊髓组织和血清中神经生长因子(NGF)蛋白表达的影响。方法174 只健康雌性Sprague-Dawley 大鼠，体质量180~220 g，随机分为正常对照组(n=54)、生理盐水(NS)对照组(n=60)和PBN组(n=60)，每时间段6 只。鞘内置管后，采用自行改制的Ò型NYU装置建立大鼠脊髓损伤模型。PBN组鞘内注射PBN 3 mg (15 μl)，NS对照组注射NS 15 μl，术后30 min 第1 次注射，连续注射7 d，每天1 次。术前3 d 和1 d，以及术后1 d、5 d、10 d、15 d、20d、25 d、30 d 和35 d 对NS对照组和PBN组进行Basso-Beattie-Bresnahan (BBB)运动功能评价，并在术后1 h、12 h、24 h、48 h、3d、7 d、14 d 和21 d 各组取血清和脊髓组织测定NGF水平。结果术后血清中NGF蛋白含量出现明显改变，而脊髓组织中含量变化不明显。血清NGF/总蛋白比值48 h 时达到峰值，NS 对照组(0.92%±0.02%)与PBN 组(0.77%±0.05%)相比有显著性差异(P=0.021)。两组大鼠BBB评分在伤后第10 天开始升高，PBN组恢复程度明显好于NS对照组(P<0.01)。结论PBN能有效降低脊髓损伤大鼠血清中NGF蛋白的表达，促进神经功能恢复。

关键词: 脊髓损伤, 自由基, N-叔丁基-α-苯基硝酮, 神经生长因子, 大鼠

Abstract: Objective To explore the effect of α-phenyl-N-tert-butylinitrone (PBN) on expression of nerve growth factor (NGF) in serum and spinal cord tissue in rats after spinal cord injury (SCI). Methods 174 female Sprague-Dawley rats were randomly assigned to following groups: normal control group (n=54), normal saline control group (NS group, n=60, intrathecally injected normal saline 15 μl), and PBN group (n=60, intrathecally injected PBN, 3 mg, 15 μl). The model was established with New York University blow device (150 kDyne, 1 s dwell time). PBN was intrathecally injected into the damaged areas 30 min after operation, then once a day for 7 days. The Basso-Beattie-Bresnahan (BBB) Locomotor Rating Scale was used to assess the rats 3 days and 1 day before, and 1 day, 5 days, 10 days, 15 days, 20 days,25 days, 30 days and 35 days after SCI. NGF in the injured spinal cord tissue and serum was measured 1 h, 12 h, 24 h, 48 h, 3 days, 7 days,14 days and 21 days after SCI. Results NGF increased in serum but not in spinal cord. The ratio of NGF/total protein in serum rose and peaked 48 h after SCI, and the ratio was higher in NS group (0.92%±0.02%) than in PBN group (0.77%±0.05%) (P=0.021). BBB scores increased from the 9th day, and PBN group improved better than NS group (P<0.01). Conclusion PBN could reduce the expression of NGF in the SCI rats, and promote the recovery of neurol function.

Key words: spinal cord injury, free radical, α-phenyl-N-tert-butylinitrone, nerve growth factor, rats