《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (2): 133-136.

• 论文 • 上一篇    下一篇

促红细胞生成素对大鼠脊髓运动神经元缺氧性损伤的保护作用

胡杨，秦文，廉凯   

  1. 湖北襄阳市中心医院(湖北文理学院附属医院)骨科，湖北襄阳市441021。
  • 收稿日期:2013-03-27 修回日期:1900-01-01 出版日期:2014-02-25 发布日期:2014-02-25
  • 通讯作者: 廉凯

Protective Effect of Erythropoietin on Anoxia of Motoneurons after Spinal Cord Injury in Rats

HU Yang, QIN Wen, LIAN Kai.   

  1. Department of Orthopedics, Central Hospital in Xiangyang, Xiangyang 441021, Hubei, China
  • Received:2013-03-27 Revised:1900-01-01 Published:2014-02-25 Online:2014-02-25

PDF (PC)

摘要： 目的观察重组红细胞生成素(EPO)对大鼠脊髓运动神经元细胞缺氧性损伤的保护作用。方法对Wistar 乳鼠脊髓运动神经元细胞进行原代分离培养，通过去除培养液中氧气来模拟脊髓缺氧。倒置显微镜下观察细胞形态变化，Western blotting 检测EPO受体(EPOR)蛋白的表达，噻唑蓝(MTT)比色法及乳酸盐脱氢酶(LDH)活力测定观察加入不同浓度EPO对神经元细胞的保护作用。结果与正常对照组比较，缺氧组脊髓运动神经元缺氧后EPOR表达及LDH浓度明显增加(P<0.01)，细胞存活率明显降低(P<0.01)；与缺氧组比较，EPO 组EPOR 表达及LDH 浓度降低(P<0.05)，细胞存活率明显增强(P<0.01)，且与浓度相关。结论EPO可减轻缺氧对运动神经元的损害，而EPOR表达上调可能是EPO发挥其对缺氧神经元保护作用的重要途径之一。

关键词: 脊髓损伤, 运动神经元, 缺氧, 促红细胞生成素, 大鼠

Abstract: Objective To observe the protective effect of erythropoietin (EPO) on anoxia of motoneurons after spinal cord injury in rats.Methods The motoneuron was dissociated from neonate Wistar rats and cultivated in vitro with deprivation of oxygen in the medium. The changes of morphology were observed by phase-contrast microscopy, and the expression of EPO receptor (EPOR) was detected with Western blotting. The survival rate of motoneuron was measured with methyl thiazolyl tetrazolium (MTT) assay, and the level of lactate dehydrogenase (LDH) was observed. Results Compared with the control group, the expression of EPOR and the level of LDH increased (P<0.01),and the survival rate of motoneuron decreased (P<0.01) in the anoxia group. Compared with the anoxia group, the expression of EPOR and the level of LDH decreased (P<0.05), and the survival rate of motoneuron increased (P<0.01) in the EPO group, which was related with the level of EPO. Conclusion EPO could relieve the damage of motoneuron caused by anoxia, especially by upregulating EPOR.

Key words: spinal cord injury, motoneuron, anoxia, erythropoietin, rats