《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (12): 1124-1127.

• 论文 • 上一篇    下一篇

银杏叶提取物抗氧化作用对大鼠脊髓损伤后运动功能恢复的影响

陈建敏，谢少华，杨拯，张艺，张晓   

  1. 成都医学院基础医学实验教学中心，四川成都市610500。
  • 收稿日期:2013-05-13 修回日期:1900-01-01 出版日期:2013-12-25 发布日期:2013-12-25
  • 通讯作者: 杨拯

Antioxidation Effect of Ginkgo Biloba Extract on Motor Function after Spinal Cord Injury in Rats

CHEN Jian-min, XIE Shao-hua,YANG Zheng, et al.   

  1. Experimental Center for Basic Medical Teaching, the School of Basic Medical Science, Chengdu Medical College,Chengdu 610500, Sichuan, China
  • Received:2013-05-13 Revised:1900-01-01 Published:2013-12-25 Online:2013-12-25

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被引次数

20

摘要： 目的观察银杏叶提取物抗氧化作用对大鼠脊髓损伤后运动功能恢复的影响。方法健康成年Sprague-Dawley 大鼠36 只，采用Allen 法(10 g×25 mm)损伤大鼠T9脊髓节段，复制脊髓损伤动物模型，并随机分为银杏叶提取物治疗组(A 组)、甲基强的松龙治疗组(B 组)和对照组(C 组)，每组12 只。造模成功后30 min，A组腹腔注射银杏叶提取物40 mg/kg，此后每天1 次；B组腹腔注射甲基强的松龙30 mg/kg，此后按5.4 mg/kg 每6 h 注射1 次，共4 次；C组每天腹腔注射等体积生理盐水。于术后1 d、3 d、7d 随机抽取各组动物4 只，进行后肢功能BBB评分及血清丙二醛(MDA)和超氧化物歧化酶(SOD)测定。结果术后各时间点A、B两组BBB评分均高于C组(P<0.05)。术后3 d、7 d，A组、B组动物体内SOD活性均高于C组(P<0.05)，而MDA含量均低于C组(P<0.05)。结论银杏叶提取物可降低脊髓损伤大鼠体内的氧化应激水平，促进脊髓损伤大鼠运动功能的恢复。

关键词: 脊髓损伤, 运动功能, 银杏叶提取物, 丙二醛, 超氧化物歧化酶, 大鼠

Abstract: Objective To observe the antioxidative effect of Ginkgo biloba extract (EGb) on motor function after spinal cord injury (SCI) in rats. Methods 36 healthy adult Sprague-Dawley rats were divided randomly into EGb group (group A), methylprednisolone (MP) group (group B) and control group (group C) with 12 rats in each group. SCI model was made by Allen's mode (10 g×25 mm) at the level of the 9th thoracic vertebra (T9). Group A was given 40 mg/kg EGb daily by intraperitoneal injection, while group B was given 30 mg/kg MP at the first injection and then 5.4 mg/kg every 6 hours for 4 times. Group C received an equal volume of normal saline daily. On the 1st, 3rd and 7th days after SCI, 4 rats were selected randomly from each group. The motor function were assessed by Basso-Beattie-Bresnahan scale (BBB). The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum were determined. Results At every time point after surgery, the BBB scores were significantly higher in groups A and B than in group C (P<0.05). Compared to group C,the activity of SOD increased and the content of MDA decreased significantly in groups A and B on the 3rd and 7th days after SCI (P<0.05).Conclusion EGb may reduce the level of oxidative stress and promote the recovery of the motor function in rats after SCI.

Key words: spinal cord injury, motor function, Ginkgo biloba extract, malondialdehyde, superoxide dismutase, rats