《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (12): 1104-1107.

• 论文 • 上一篇    下一篇

电针对骶髓损伤后神经源性膀胱大鼠尿流动力学及膀胱组织中

艾坤，张泓，张雨辰，鲍秋影，李景兴，匡静之   

  1. 湖南中医药大学针灸推拿学院，湖南长沙市410007。
  • 收稿日期:2013-08-08 修回日期:2013-12-04 出版日期:2013-12-25 发布日期:2013-12-25
  • 通讯作者: 张泓

Effect of Electrical Acupuncture on Urodynamics and Expression of Caspase-3 in Bladder Tissue in Rats with Neurogenic Bladder after Sacral Spinal Sord Injury

AI Kun, ZHANG Hong, ZHANG Yu- chen, et al   

  1. Hunan University of Traditional Chinese Medicine,Changsha 410007, Hunan, China
  • Received:2013-08-08 Revised:2013-12-04 Published:2013-12-25 Online:2013-12-25

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摘要： 目的观察电针次髎、中极、三阴交穴对骶髓损伤后神经源性膀胱(逼尿肌无反射型)大鼠尿流动力学以及膀胱组织中caspase-3 表达的影响。方法雌性Sprague-Dawley大鼠40 只，随机抽取10 只为空白组，其余造模后随机分为模型组、穴位组和对照点组，每组10 只。术后第15 天起按组分别施以7 次治疗。测量术后第14 天及干预治疗7 次后1 d 的大鼠尿流动力学参数；治疗结束后取膀胱组织采用ELISA法检测在膀胱组织中caspase-3 的表达。结果电针治疗后大鼠最大膀胱容量显著降低(P<0.001)，膀胱顺应性显著降低(P<0.001)，逼尿肌组织中caspase-3 表达明显降低(P<0.01)，充盈性膀胱内压升高(P<0.05)，且穴位组疗效优于对照点组(P<0.05)。结论电针次髎、中极、三阴交穴可改善骶髓损伤后神经源性膀胱大鼠尿流动力学参数，降低膀胱组织中caspase-3 的表达。

关键词: 脊髓损伤, 逼尿肌无反射, 电针, 尿流动力学, caspase-3, 大鼠

Abstract: Objective To explore the effects of electrical acupuncture at Ciliao (BL32), Zhongji (RN03), Sanyinjiao (SP06) acupoints on the expression of caspase-3 in bladder tissue and the urodynamics of rats with neurogenic bladder (detrusor areflexia type) after sacral spinal cord injury. Methods 10 rats were randomly selected as blank control group from 40 female Sprague-Dawley rats. The other 30 rats were randomly divided into model group, acupoint group and control group after modeling, with 10 rats in each group. The urodynamics was measured on the 14th and 22nd day after modeling. The control group and acupoint group received electrical acupuncture 7 times at the acupoints and other points nearby, respectively, from the 15th day. The expression of caspase- 3 in bladder tissue was detected with enzyme-linked immuno sorbent assay (ELISA) method. Results Compared with the model group, the maximum bladder capacity, bladder compliance and expression of caspase-3 in bladder tissue significantly decreased after treatment (P<0.01), the filling bladder pressure significantly increased (P<0.05) in the acupoint group. And the acupuncture group was better than the control group (P<0.05). Conclusion Electrical acupuncture at Ciliao, Zhongji and Sanyinjiao can reduce the maximum bladder capacity in neurogenic bladder rats and inhabit the expression of caspase-3 in bladder tissue.

Key words: spinal cord injury, detrusor areflexia, electrical acupuncture, urodynamics, caspase-3, rats