《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (10): 922-926.

• 论文 • 上一篇    下一篇

三七总皂苷对大鼠脊髓半横断损伤后的神经保护作用

皮斌，孙扬，熊巍，沈田，周志扬   

  1. 1.苏州大学附属第一医院骨科，江苏苏州市215021；2.中南大学湘雅医学院，湖南长沙市410001。
  • 收稿日期:2013-04-09 修回日期:2013-09-11 出版日期:2013-10-25 发布日期:2013-10-25

Protective Effect of Panax Notoginseng Saponins in Spinal Cord Hemisection Injured Rats

PI Bin, SUN Yang, XIONG Wei, et al.   

  1. Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou 215021, Jiangsu, China
  • Received:2013-04-09 Revised:2013-09-11 Published:2013-10-25 Online:2013-10-25

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被引次数

8

摘要： 目的探讨三七总皂苷(PNS)对大鼠脊髓半横断损伤后继发性损害的神经保护作用及相关机制。方法55 只成年Sprague-Dawley 大鼠随机分成3 组：假手术组(n=5)、脊髓损伤组(n=25)和PNS组(n=25)。造模成功后1 d、3 d、7 d、14 d 和21 d 应用BBB评分标准对大鼠后肢运动功能进行行为学评分，相应时间点取材后对大鼠脊髓组织进行病理形态学观察和免疫组化分析。结果PNS干预后3 d、7 d、14 d 和21 d 时PNS组大鼠运动功能恢复优于脊髓损伤组(P<0.05)。7 d、14 d 和21 d 时PNS组尼氏染色示神经元坏死减少，形态完整，且胞浆型磷脂酶A2(cPLA2)阳性细胞数减少，神经元形态完整，cPLA2 表达增强被抑制。结论PNS可通过抑制cPLA2 表达在脊髓损伤中发挥神经保护作用，促进运动功能恢复。

关键词: 三七总皂苷, 脊髓损伤, 胞浆型磷脂酶A2, 大鼠

Abstract: Objective To investigate the protective effect and cytosolic phospholipase A2 (cPLA2) associated mechanism of panax notoginseng saponins (PNS) in spinal cord hemisection injured rats. Methods 55 adult Sprague-Dawley rats were randomly divided into three groups: sham group (n=5), spinal cord injury group (n=25) and PNS group (n=25). The rats were evaluated with BBB score, pathology and immunohistochemistry 1 d, 3 d, 7 d, 14 d and 21 d after intervention. Results Compared to the spinal cord injury group, motor recovery was significantly better in PNS group 3 d, 7 d, 14 d and 21 d after intervention (P<0.05). Nissl staining showed less neuron necrosis and more integrated neural cells in morphology in PNS group 7 d, 14 d and 21 d after intervention. Cytosolic phospholipase A2 (cPLA2) expression was inhabited, and less number of positive cells were found in PNS group 7 d, 14 d and 21 d after intervention. Conclusion PNS can inhibit the expression of cPLA2 after spinal cord injury, which may be one of the mechanisms of its effect on promoting motor recovery.

Key words: panax notoginseng saponins, spinal cord injury, cytosolic phospholipase A2, rats