《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (08): 739-742.

• 论文 • 上一篇    下一篇

慢性间断性低氧对大鼠海马神经元及生长相关蛋白的进行性影响

陈燕，赵春玲，罗礼容，余广   

  1. 1.泸州医学院，a. 生理学教研室；b.机能实验中心，四川泸州市646000。
  • 收稿日期:2013-01-23 修回日期:2013-03-06 出版日期:2013-08-25 发布日期:2013-08-25

Progressive Effects of Chronic Intermittent Hypoxia on Hippocampus Neuron and Growth Associated Protein-43 in Rats

CHEN Yan, ZHAO Chun-ling, LUO Li-rong, et al.   

  1. Department of Physiology, Center of Functional Experiment, Luzhou Medical College, Luzhou 646000, Sichuan, China
  • Received:2013-01-23 Revised:2013-03-06 Published:2013-08-25 Online:2013-08-25

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摘要： 目的观察慢性间断性低氧(CIH)过程中大鼠海马神经元的组织结构及生长相关蛋白(GAP)-43 表达的进行性变化。方法成年雄性Sprague-Dawley 大鼠40 只，随机分为对照组、CIH 1 周组、CIH 3 周组和CIH 5 周组，每组各10 只。后三组大鼠在自制缺氧舱内每天缺氧8 h，连续缺氧1 周、3 周和5 周。HE染色后在光学显微镜下观察海马神经元组织结构；免疫组化方法检测海马神经元GAP-43 的表达。结果CIH大鼠海马出现变性坏死神经元，并且随着缺氧时间延长，上述改变呈逐渐加重趋势。与对照组比较，CIH 组大鼠海马神经元GAP-43 阳性表达增多，CIH 后1 周出现表达高峰(P=0.038)，CIH 后3 周表达逐渐减少(P=0.382)，CIH后5 周表达接近正常水平(P=0.860)。结论随着缺氧时间延长，变性坏死神经元数量呈进行性增多，而GAP-43 的表达却与此不一致。

关键词: 慢性间断性低氧, 生长相关蛋白, 海马, 大鼠

Abstract: Objective To explore the effect of chronic intermittent hypoxia (CIH) on hippocampus neuron and growth associated protein-43 (GAP-43) in rats. Methods 40 adult male Sprague-Dawley rats were randomly divided into control group, CIH 1 week group, CIH 3 weeks group and CIH 5 weeks group with 10 rats in each group. The CIH groups were exposed to intermittent hypoxia in designed cabin 8 hevery day for 1 week, 3 weeks and 5 weeks. The pathological changes of hippocampal neurons were observed by HE staining. The GAP-43 expression of hippocampus was detected by immunohistochemical staining. Results There was degeneration and necrosis of hippocampal neurons in the CIH rats, and it became worse gradually along with the hypoxia. Compared with the control group, the GAP-43 expression in hippocampus increased in CIH rats. The peak of GAP-43 expression appeared in the 1st week (P=0.038) and then it decreased in the 3rd week (P=0.382) and it was closed to the normal level in the 5th week (P=0.860). Conclusion Degeneration and necrosis of hippocampal neurons exacerbated gradually along with the hypoxia, while GAP-43 expression changed inconsistently.

Key words: chronic intermittent hypoxia, growth associated protein, hippocampus, rats