《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (07): 628-630.

• 论文 • 上一篇    下一篇

咯利普兰治疗大鼠脊髓横断损伤的实验研究

李锋涛，程斌，李浩鹏，臧全金，张廷，贺西京   

  1. 1.西安交通大学第二附属医院，a.骨二科；b.骨三科，陕西西安市710004。
  • 收稿日期:2012-07-19 修回日期:2012-11-28 出版日期:2013-07-25 发布日期:2013-07-25
  • 通讯作者: 贺西京

Effects of Rolipram on Spinal Cord Transection Injury in Rats

LI Feng-tao, CHENG Bin, LI Hao-peng, et al.   

  1. Department of Orthopedics,the Second Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi, China
  • Received:2012-07-19 Revised:2012-11-28 Published:2013-07-25 Online:2013-07-25

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摘要： 目的探索咯利普兰治疗大鼠脊髓横断损伤的可行性。方法取健康成年雌性Sprague-Dawley 大鼠30 只，随机分为假手术组(Sham组)、损伤组(SCI 组)、咯利普兰治疗组(R 组)，每组10 只。R 组建立大鼠脊髓横断损伤模型后立刻皮下注射咯利普兰；Sham组仅打开椎板；SCI 组及Sham组皮下注射相同体积二甲基亚砜(DMSO)。于损伤后2、4、6、8 周采用BBB评分法观察大鼠后肢运动功能情况，损伤后2 周时应用免疫组织化学染色检测各组生长相关蛋白-43(GAP-43)及胶质细胞酸性蛋白(GFAP)的表达。结果损伤后6 周、8 周时，R 组BBB 评分优于SCI 组(P<0.05)。损伤后2 周，R 组GAP-43 表达高于SCI 组(P<0.05)，GFAP 表达量低于SCI 组(P<0.05)。结论咯利普兰能提高大鼠脊髓横断损伤神经功能评分，提高GAP-43 表达，抑制GFAP 表达。

关键词: 脊髓损伤, 咯利普兰, 运动功能, 生长相关蛋白43, 胶质细胞酸性蛋白, 大鼠

Abstract: Objective To investigate the possibility of rolipram for treatment of spinal cord injury (SCI) in rats. Methods 30 adult female Sprague-Dawley rats were divided into sham-operation group (sham group, n=10), spinal cord injury group (SCI group, n=10) and rolipram treatment group (R group, n=10). The rats in SCI group and R group were modeled as spinal cord transection injury, and R group was administrated with rolipram subcutaneouly after SCI. They were assessed with Basso Beattie and Bresnahan (BBB) score 2, 4, 6, and 8 weeks after SCI, and the expressions of growth associated protein 43 (GAP-43) and glial fibrillary acidic protein (GFAP) were detected with immunohistochemistry 2 weeks after SCI. Results There were significant difference in the BBB scores between SCI and R groups 6 and 8 weeks after SCI (P<0.05). The expression of GAP-43 was more and GFAP was less in R group than in SCI group (P<0.05). Conclusion Rolipram can increase the expression of GAP-43 and inhibit the expression of GFAP, while improves the the motor function in rats after spinal cord transsection injury.

Key words: spinal cord injury, rolipram, motor function, growth associated protein 43, glial fibrillary acidic protein, rats