《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (06): 532-535.

• 论文 • 上一篇    下一篇

塞来昔布对神经胶质瘤细胞U251 增殖及凋亡的影响

冯晨，冯华，温宏丽，刘劲睿   

  1. 1.牡丹江医学院，a：红旗医院普外科；b：病理生理学教研室；c：外语教研室，黑龙江牡丹江市157011；2.佳木斯大学附属第一医院神经外科，黑龙江佳木斯市154002。
  • 收稿日期:2013-03-01 修回日期:2013-04-19 出版日期:2013-06-25 发布日期:2013-06-25
  • 通讯作者: 刘劲睿

Effect of Celecoxib on Proliferation and Apoptosis of U251 Glioma Cells

FENG Chen, FENG Hua, WEN Hong-li, et al.   

  1. Department of Neurosurgery, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang, China
  • Received:2013-03-01 Revised:2013-04-19 Published:2013-06-25 Online:2013-06-25

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摘要： 目的探讨非甾体类抗炎药(NSAIDs)对神经胶质瘤细胞增殖的影响。方法选用神经胶质瘤细胞U251，分别加入0μmol/L、10 μmol/L、20 μmol/L、40 μmol/L和80 μmol/L的塞来昔布进行处理。四甲基偶氮唑盐比色(MTT)法检测U251 细胞增殖水平及抑制率，流式细胞术检测细胞凋亡率。结果随塞来昔布浓度的增加，神经胶质瘤细胞U251 增殖水平明显降低，增值抑制率升高。不同浓度和不同作用时间，塞来昔布对U251 细胞增殖抑制率有显著性差异(P<0.05)；流式细胞术细胞凋亡率检测显示，80μmol/L 塞来昔布处理48 h 的U251 细胞凋亡率(17.86%)较0 μmol/L (11.23%)增高(P<0.05)。结论塞来昔布可抑制人胶质瘤细胞U251 的生长和增殖，促进U251 的凋亡发生，以80 μmol/L为佳。

关键词: 胶质瘤, U251 细胞, 塞来昔布, 增殖, 凋亡

Abstract: Objective To explore the effect of celecoxib on proliferation and apoptosis of the glioma cells. Methods The glioma cell U251 was used and disposed with different densities of celecoxib (0 μmol/L, 10 μmol/L, 20 μmol/L, 40 μmol/L and 80 μmol/L) for 24 h, 48h and 72 h. The methyl thiazolyl tetrazolium (MTT) assay was used to detect the tumor cell proliferation and flow cytometry was used to detect the tumor cell apoptosis rate. Results The Glioma U251 cells proliferation were significantly decreased with the increase of density of celecoxib in vitro, and there was significant difference in the inhibited rate in different density and different time (P<0.05). The apoptosis rate was higher in the density of 80 μmol/L (17.86%) than in that of 0 μmol/L (11.23%) (P<0.05). Conclusion Celecoxib can inhabit the proliferation of glioma U251 cells, and promote the apoptosis especially with the density of 80 μmol/L.

Key words: glioma, U251 cell, celecoxib, proliferation, apoptosis