《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (05): 436-439.

• 论文 • 上一篇    下一篇

早期干预对宫内感染致脑损伤仔鼠脑组织NB-3 表达的影响

袁新华，王红丽，邓庆先   

  1. 湖州市妇幼保健院儿科，浙江湖州市313000。
  • 收稿日期:2012-10-18 修回日期:2012-12-14 出版日期:2013-05-25 发布日期:2013-05-25

Effect of Early Intervention on Expression of NB-3 in Neonatal Rats with Cerebral Injury Induced with Intrauterine Infection

YUAN Xin-hua, WANG Hong-li, DENG Qing-xian.   

  1. Department of Pediatrics, Huzhou Maternity & Child Care Hospital, Huzhou 313000, Zhejiang,China
  • Received:2012-10-18 Revised:2012-12-14 Published:2013-05-25 Online:2013-05-25

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摘要： 目的探讨早期干预对仔鼠脑组织NB-3 的表达及其运动功能的影响。方法孕17~18 d Wistar 大鼠45 只腹腔注射脂多糖(LPS) 380 μg/kg 连续2 d (LPS 组)，另15 只注射同等剂量的无菌生理盐水(NS 组)。随机选取LPS 组仔鼠80 只，分为干预组40只(I 组)和非干预组40 只(NI 组)；随机选取NS 组仔鼠40 只为对照组。早期干预措施主要给予早期触摸与丰富康复训练。仔鼠出生后24 h 内在LPS 组和NS 组随机取5 只行脑组织HE 染色。其余大鼠分别于出生后14 d、28 d 行悬吊试验，1 d、7 d、14 d、21 d、28 d 行脑组织免疫组织化学染色，观察NB-3 的表达。结果脑组织NB-3 的表达及悬吊试验评分三组间均有非常高度显著性差异(P<0.001)。结论宫内感染可致脑损伤鼠NB-3 表达增加，早期干预使脑损伤鼠NB-3 表达持续增加，并改善其运动功能。

关键词: 宫内感染, 脑损伤, 早期干预, NB-3, 神经可塑性, 大鼠

Abstract: Objective To investigate the effect of early intervention on adhesion molecule NB-3 expressing in cerebral injured neonatal rat induced with intrauterine infection. Methods Pregnant rats were consecutively injected with lipopolysaccharide (LPS) 380 μg/kg or saline 17 d after gestation. The offspring from the LPS group accepted early intervention (I group, n=40) or not (NI group, n=40), and those for saline group were as control group (n=40). They were assessed with suspension test 14 d and 28 d after born, and the expression of were NB-3 were detected with immunohistochemistry 1 d, 7 d, 14 d, 21 d, 28 d after born. Results The expression of NB-3 was different among these 3 groups, as well as the scores of suspension test (P<0.001). Conclusion Intrauterine infection increases the expression of NB-3 of neonatal rats with brain damage. The early intervention may increase persistently the expression of NB-3, and improve the motor function.

Key words: intrauterine infection, cerebral injury, early intervention, NB-3, neuronal plasticity, rats