《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (05): 432-436.

• 论文 • 上一篇    下一篇

丙戊酸钠诱导孤独症模型大鼠皮层Bcl-2、Bax 的表达

姜志梅,崔利军，郭津，张士岭，郭岚敏   

  1. 1.佳木斯大学，a.第三附属医院儿童保健科；b.康复医学院；c.儿童神经康复实验室，黑龙江佳木斯市154007。
  • 收稿日期:2013-03-10 修回日期:2013-04-04 出版日期:2013-05-25 发布日期:2013-05-25

Expression of Bcl-2 and Bax in Cerebral Cortex of Sodium Valproate Induced Autism Rats

JIANG Zhi-mei, CUI Li-jun, GUO Jin, et al   

  1. The Third Affiliated Hospital of Jiamusi University, Jiamusi 154007, Heilongjiang, China
  • Received:2013-03-10 Revised:2013-04-04 Published:2013-05-25 Online:2013-05-25

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摘要： 目的探讨Bcl-2、Bax 在孤独症发病中的作用。方法孕12.5 d Sprague-Dawley 孕鼠腹腔注射丙戊酸钠600 mg/kg 建立子代孤独症模型大鼠，对照组注射同等剂量生理盐水。利用尼氏染色、免疫组化和图像分析技术观察比较出生后1 d 、7 d 、14 d、28 d 和56 d 两组大鼠脑部Bcl-2、Bax 表达。结果尼氏染色：出生后1 d、7 d 模型组神经元数量较少，出生14 d 后剧增，出生后28 d、56 d 仍高于对照组。免疫组化：出生后1~14 d 两组Bcl-2、Bax 表达均显著升高(P<0.001)，出生28 后表达趋于稳定(P>0.05)；与对照组相比，模型组各日龄大鼠Bcl-2 表达水平均显著降低(P<0.001)；Bax 于出生后1 d、7 d 表达显著升高(P<0.001)，出生后14 d 表达显著降低(P<0.001)，出生28 d 后水平相似(P>0.05)。对照组Bcl-2/Bax 值出生后1 d 最大，随年龄逐渐减小，出生14后趋近于1。模型组Bcl-2/Bax 值出生后7 d 最低，14 d 最高，28 d 后趋于稳定。结论孤独症模型大鼠大脑皮层神经元细胞抗凋亡能力下降，尤其在出生后早期。

关键词: 孤独症, 凋亡, Bcl-2, Bax, 大鼠

Abstract: Objective To explore the role of Bcl-2 and Bax in pathogenesis of the autism. Methods Female Sprague-Dawley rats were given a single intraperitoneal injection of sodium valproate (VPA, 600 mg/kg) on 12.5 d after pregnancy, their offspring were as the model group; while the other pregnancy rats were given normal saline, their offspring were as the control group. Both groups were observed with the Nissl staining, immunohistochemistry of Bcl-2 and Bax and image analysis 1 d, 7 d, 14 d, 28 d, 56 d after birth. Results Compared with the control group, Nissl staining showed the number of cortical neurons decreased on 1 d and 7 d after birth in the model group, rapidly increased on 14 d after birth, and maintained in high level on 28d , 56 d after birth. For immunohistochemistry, the integrated optical density (IOD) of Bcl-2 and Bax decreased in cortex on 1~14 d after birth (P<0.001) in both groups, and were stable 28 d after birth (P>0.05). Compared with the control group, the IOD of Bcl-2 decreased much more at every time point (P<0.001) in the model group, but the IOD of Bax increased on 1 d, 7 d (P<0.001), decreased on 14 d (P<0.001), similar 28 after birth(P>0.05). The ratio of Bcl-2/Bax was the most on 1 d after birth, and then decreased to approximately 1 in the control group, while it was the least on 7 d, most on 14 d, and decrease to less than 128 d after birth. Conclusion Apoptosis of cerebral cortex neurons increases in the autism model rats, especially in the early time.

Key words: autism, apoptosis, Bcl-2, Bax, rats