《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (1): 42-45.

• 论文 • 上一篇    下一篇

脑源性神经营养因子对持续惊厥后海马凋亡调控基因表达的影响

苑爱云，蒋莉，侯梅，李欣   

  1. 1.青岛市妇女儿童医疗保健中心神经康复科，山东青岛市266034；2.重庆医科大学附属儿童医院神经内科，重庆市400014。
  • 收稿日期:2012-06-08 修回日期:2012-07-11 出版日期:2013-01-25 发布日期:2013-01-25
  • 通讯作者: 蒋莉

Effects of Brain-derived Neurotrophic Factor on Apoptotic Regulated Genes in Hippocampal Cells after Status Convulsivus

YUAN Ai-Yun, JIANG Li, HOU Mei, et al.   

  1. Department of Neurology & Rehabilitation, Qingdao Women & Children Medical Healthacre Center,Qingdao 266034, Shandong, China
  • Received:2012-06-08 Revised:2012-07-11 Published:2013-01-25 Online:2013-01-25

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摘要： 目的观察外源性脑源性神经营养因子(BDNF)对惊厥持续状态(SC)后海马凋亡调控基因表达的影响。方法选用成年Wistar 鼠32 只制作氯化锂-匹罗卡品SC模型，另外32 只为正常对照组。两组分别于一侧脑室内注射生理盐水、BDNF、抗BDNF抗体或不注射，于注射后6 h 处死。免疫组化观察海马Bcl-2 和c-Jun 蛋白的表达；原位杂交和RT-PCR 测定bcl-2 和c-jun mRNA 的表达。结果正常对照组双侧海马bcl-2 及c-jun 表达与同组无注射组比较无显著性差异。SC 组注射侧海马bcl-2 表达由高而低依次为：SC-BDNF 组>SC-无注射组=SC-NS 组(P<0.05)>SC-抗BDNF 组(P<0.05)，c-jun 表达则呈相反变化趋势(P<0.05)；SC 组注射对侧海马bcl-2 及c-jun 表达与无注射对照组相比无显著性差异。结论SC 后，脑室内注射外源性BDNF 可能通过上调凋亡抑制基因bcl-2、下调凋亡促进基因c-jun 表达发挥其保护作用。脑室内注射BDNF 在脑内扩散有限，在临床的应用受限。

关键词: 惊厥持续状态, 脑源性神经营养因子, 凋亡, bcl-2, c-jun, 大鼠

Abstract: Objective To explore the effects of brain-derived neurotrophic factor (BDNF) on expression of apoptotic regulated genes (bcl-2 and c-jun) in hippocampus after status convulsivus (SC). Methods Seizures were induced in 32 adult Wistar rats with lithium-pilocarpine intraperitoneal injection (SC), the other 32 rats were as the normal controls (NC). The rats were sacrificed 6 h after injection of normal saline (NS), BDNF, or anti-BDNF in left lateral ventricle (or no injection). The expression of Bcl-2 and c-Jun protein and bcl-2 and c-jun mRNA were investigated with immunocytochemistry, RT-PCR and in situ hybridization. Results The expression of bcl-2 and c-jun (both protein and mRNA) was not significantly different in the hippocampus of both side in NC. In SC, the expression of bcl-2 ranged from more to less as BDNF>NS and non-injection>anti-BDNF in the hippocampus of non-injected side, while the expression of c-jun reversed. However,there was not significant difference in the expression of bcl-2 or c-jun in the hippocampus of non-injected side. Conclusion Exogenous BDNF can up-regulate the expression of bcl-2 and down-regulate the expression of c-jun in hippocampal cells, which may protect brain from apoptosis after after SC. The intraventricular injection of BDNF diffuses limited, which works less for clinical treatment.

Key words: status convulsivus, brain-derived neurotrophic factor, apoptosis, bcl-2, c-jun, rats