《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (2): 132-135.

• 论文 • 上一篇    下一篇

番茄红素抗氧化应激效应对大鼠脊髓损伤后运动功能恢复的影响

李晓，罗晨禹，杨拯，谢少华，张尧，杨海燕，马延仃，张艺，胡雯思，卓睿，李亚光   

  1. 成都医学院，四川成都市610081。
  • 收稿日期:2012-07-30 修回日期:1900-01-01 出版日期:2013-02-25 发布日期:2013-02-25
  • 通讯作者: 杨拯，张晓

Anti-oxidative Stress Effect of Lycopene on Motor Function after Spinal Cord Injury in Rats

LI Xiao, LUO Chen-yu, YANG Zheng,et al.   

  1. Chengdu Medical College, Chengdu 610081, Sichuan, China
  • Received:2012-07-30 Revised:1900-01-01 Published:2013-02-25 Online:2013-02-25

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被引次数

26

摘要： 目的观察番茄红素抗氧化应激效应对大鼠脊髓损伤后运动功能恢复的影响。方法健康成年Sprague-Dawley 大鼠36 只，采用Allen 法(10 g×25 mm)在T9造成大鼠脊髓损伤模型，并随机分为对照组(A组)、甲泼尼龙琥珀酸钠(MP)治疗组(B 组)和番茄红素治疗组(C 组)，每组12 只。造模成功30 min 后，A组不给予治疗，B 组腹腔注射MP 30 mg/kg，C 组灌胃番茄红素20 mg/kg。于术后1 d、3 d、7 d 分别对各组进行斜板试验、后肢功能BBB评分、血清中的超氧化物歧化酶(SOD)和丙二醛(MDA)测定。结果与A组相比，C组1 d、3 d 和B组1 d、3 d、7 d 斜板试验评分升高(P<0.05)；C组1 d、3 d、7 d 和B组1 d、7 d BBB评分升高(P<0.05)；C组1 d、3 d、7 d 和B组3 d、7 d SOD活性升高(P<0.05)；C组3 d、7 d 和B组1 d、7 d MDA含量降低(P<0.05)。结论番茄红素可以降低急性脊髓损伤后的氧化应激水平，促进大鼠运动功能恢复。

关键词: 番茄红素, 脊髓损伤, 丙二醛, 超氧化物歧化酶, 运动功能

Abstract: Objective To observe the influence of anti-oxidative stress effect of lycopene (LP) on motor function after spinal cord injury (SCI) in rats. Methods 36 healthy adult Sprague-Dawley rats were randomly divided into Groups A, B and C with 12 rats in each group.SCI model was made by Allen's mode (10 g×25 mm) on T9. Group A received no treatment, 30 mg/kg methylprednisolone sodium succinate was injected for Group B in peritoneal cavity 30 min after SCI, 20 mg/kg LP was given orally for Group C. On the 1st d, 3rd d and 7th d after operation, the motor function was assessed by slanting board test and Basso-Beattie-Bresnahan scale (BBB); superoxide dismutase (SOD) and malondialdehyde (MDA) were determined in serum. Results Compared with Group A, the scores of the slanting board test significantly increased in Group C 1 d, 3 d, and in Group B 1 d, 3 d and 7 d after operation (P<0.05); the BBB scores significantly increased in Group C 1 d, 3 d and 7 d, and in Group B 1 d, 7 d after operation (P<0.05); the activity of SOD significantly increased in Group C 1 d, 3 d and 7 d, and in Group B 3 d, 7 d after operation (P<0.05); the content of MDA significantly decreased in Group C 3 d, 7 d, and in Group B 1 d, 7 d after operation (P<0.05). Conclusion Lycopene can reduce the level of oxidative stress and promote the motor function in rats after acute SCI.

Key words: lycopene, spinal cord injury, malondialdehyde, superoxide dismutase, motor function