《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (2): 114-118.

• 论文 • 上一篇    下一篇

电针对大鼠局灶性脑缺血再灌注后脑内血管内皮生长因子受体-2 mRNA和脑源性神经营养因子蛋白表达的影响

马璟曦，罗勇，蔡敏   

  1. 1.重庆市中山医院，重庆市400013；2.重庆医科大学附属第一医院神经内科，重庆市神经病学重点实验室，重庆市400016。
  • 收稿日期:2012-08-20 修回日期:2012-10-24 出版日期:2013-02-25 发布日期:2013-02-25
  • 通讯作者: 罗勇

Effect of Electroacupuncture on Expression of Vascular Endothelial Growth Factor Receptor-2 mRNA and Brain-Derived Neurotrophic Factor Protein in Brain Tissue of Rats with Focal Cerebral Ischemia/Reperfusion

MA Jing-xi, LUO Yong, CAI Min   

  1. Chongqing Zhongshan Hospital, Chongqing 400013, China
  • Received:2012-08-20 Revised:2012-10-24 Published:2013-02-25 Online:2013-02-25

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被引次数

18

摘要： 目的观测电针大鼠双侧合谷穴(LI 4)对局灶性脑缺血再灌注后脑内血管内皮生长因子受体-2(VEGFR-2) mRNA和脑源性神经营养因子(BDNF)蛋白表达的影响，探讨神经发生和血管生成间可能的相互联系和作用。方法线栓法制备大鼠局灶性脑缺血再灌注模型。90 只大鼠随机分为对照组(n=6)、模型组(n=42)和电针组(n=42)。观察局灶性脑缺血1 h 后再灌注2 h、12 h、24h、3 d、7 d、14 d、21 d 共7 个时相点，每个时相点6 只大鼠。采用反转录酶-聚合酶链锁反应(RT-PCR)法检测VEGFR-2 mRNA的表达，免疫组化法检测BDNF蛋白的表达。结果再灌注后12 h、24 h，电针组VEGFR-2 mRNA明显高于模型组(P<0.01)，3 d 也高于模型组(P<0.05)。与模型组比较，电针组海马区BDNF蛋白在再灌注后2 h 开始增加(P<0.05)，24 h 达高峰(P<0.01)，3 d 后仍有显著性差异(P<0.05)，但呈现增加幅度降低的趋势。结论电针能促进脑缺血再灌注后VEGFR-2 mRNA、BDNF蛋白的表达；神经血管因子可能是神经发生与血管生成联系与作用的分子学基础。

关键词: 缺血/再灌注, 神经血管因子, 血管内皮生长因子受体-2, 脑源性神经营养因子, 电针, 大鼠

Abstract: Objective To observe the effect of electroacupuncture on the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) mRNA and brain-derived neurotrophic factor (BDNF) protein in brain tissue of rats with focal cerebral ischemia/reperfusion.Methods The focal cerebral ischemia/reperfusion model was established with filament occlusion. 90 rats were randomly divided into control group (n=6), model group (n=42) and electroacupuncture group (n=42). They were observed 2 h, 12 h, 24 h, 3 d, 7 d, 14 d, 21 d after ischemia/reperfusion, 6 rats in each points. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of VEGFR-2 mRNA. Immunohistology method was used to detect the expression of BDNF protein. Results The expression of VEGFR-2 mRNA was higher in the electroacupuncture group than in the model group 12 h, 24 h, 3 d after reperfusion (P<0.05). The expression of BDNF protein in the hippocampus increased 2 h (P<0.05), reached peak 24 h (P<0.01) and still higher 3 d (P<0.05) in the electroacupuncture group than in the model group after reperfusion. Conclusion Electroacupuncture can upregulate the expression of VEGFR-2 mRNA and BDNF protein in brain tissue of rats with focal cerebral ischemia/reperfusion. Neuro-angiogenic factor is possibly the molecular basis of coordinated interaction of the nervous and vascular system.

Key words: ischemia/reperfusion, neuro-angiogenic factors, vascular endothelial growth factor receptor-2, brain-derived neurotrophic factor, electroacupuncture, rats