《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (5): 417-419.

• 论文 • 上一篇    下一篇

神经干细胞-施万细胞-聚乳酸-羟基乙酸共聚物支架移植对脊髓损伤大鼠的影响

夏雷1，郝淑煜2，李德志2，陈刚3，高川川1，历俊华4，万虹4   

  1. 1.北京三博脑科医院，首都医科大学第十一临床学院，北京市 100093；2.首都医科大学附属北京天坛医院，北京市 100050；3.无锡市第四人民医院神经外科，江苏无锡市 214062；4.首都医科大学附属北京市神经外科研究所，北京市 100050。
  • 收稿日期:2012-02-20 修回日期:2012-03-28 出版日期:2012-05-25 发布日期:2012-05-25

Effect of Complex of Neural Stem Cells, Schwann Cells, and Poly (Lactic-co-glycolic Acid) Scaffolds Transplant on Spinal Cord Injured Rats

XIA Lei, HAO Shu-yu, LI De-zhi, et al.   

  1. Beijing Sanbo Brain Hospital, Capital Medical University, Beijing 100093, China
  • Received:2012-02-20 Revised:2012-03-28 Published:2012-05-25 Online:2012-05-25

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被引次数

7

摘要： 目的探讨种植神经干细胞(NSCs)与施万细胞(SCs)的聚乳酸-羟基乙酸共聚物(PLGA)支架移植促进脊髓损伤大鼠神经功能恢复的作用及机制。方法体外培养NSCs 和SCs，以PLGA 为支架移植入大鼠T8 半横断脊髓损伤处。实验动物随机分为PLGA组、PLGA+NSCs 组和PLGA+NSCs+SCs 组。术前和术后进行皮层运动诱发电位(CMEPs)检查及BBB评分；然后在同侧或对侧进行T6再次半横断，并进行CMEPs 检测及BBB 评分。结果CMEPs 的恢复率及波幅在PLGA+NSCs+SCs 组最高。移植后，大鼠BBB 评分逐渐改善；在移植后第2 周及以后，PLGA+NSCs 组和PLGA+NSCs+SCs 组的BBB 评分显著高于PLGA 组(P<0.001)。同侧再次半横断后，CMEPs 消失， BBB 评分快速恢复；对侧再次半横断后，大鼠双下肢完全瘫痪。结论种植NSCs 和SCs 的PLGA支架移植有利于脊髓损伤功能重建，再生轴突可能形成了功能性连接；但是同侧再生轴突对脊髓功能的恢复作用有限。

关键词: 脊髓损伤, 组织工程, 神经干细胞, 施万细胞, 聚乳酸-羟基乙酸共聚物, 支架

Abstract: Objective To explore the effect of transplanting poly (lactic-co-glycolic acid) (PLGA) scaffolds seeded with neural stemcells (NSCs) and Schwann cells (SCs) on spinal cord injured rats and the mechanism. Methods NSCs and SCs were cultured in vitro andthen seeded into the directional PLGA scaffolds. Then PLGA-cell complexes were implanted into the spinal cord hemisected rats, whichwere divided into PLGA group, PLGA+NSCs group and PLGA+ NSC+SCs group. The rats were tested with cortical motor evoked potentials(CMEPs) and Basso-Beattle-Bresnahan (BBB) score. Then, the rats were further ipsilaterally or contralateral hemisected at T6 and testedwith CMEPs and BBB score again. Results The incidence of recovery and the amplitudes of CMEPs were the highest in PLGA+NSCs+SCs group. The rats exhibited a gradual improvement in hindlimb locomotor function in score. The BBB score was the least in the PLGAgroup in the 2nd week or later. After retransected ipsilaterally, the CMEPs disappeared again and the BBB score improved quickly. But afterretransected contralaterally, the rats were completely paraplegia. Conclusion The directional PLGA scaffolds seeded with NSCs and SCs facilitatethe recovery in spinal cord injured rats, which may associate with axonal regeneration and functional connections, but play a limitedrole.

Key words: spinal cord injury, tissue engineering, neural stem cells, Schwann cells, poly (lactic-co-glycolic acid), scaffold