《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (5): 411-413.

• 论文 • 上一篇    下一篇

超短波对大鼠脑缺血再灌注后肿瘤坏死因子-α和Bcl-2 表达的影响

黄雄昂，叶祥明，林坚，刘晓林，李琳   

  1. 浙江省人民医院康复医学科，浙江杭州市 310014。
  • 收稿日期:2012-01-16 修回日期:2012-04-06 出版日期:2012-05-25 发布日期:2012-05-25

Effect of Ultrashort Wave Diathermy on Expression of Tumor Necrosis Factor-α and Bcl-2 after Cerebral Ischemia/Reperfusion inRats

HUANG Xiong-ang, YE Xiang-ming, LIN Jian, et al.   

  1. Department of Rehabilitation Medicine, Zhejiang Provincial People's Hospital,Hangzhou 310014, Zhejiang, China
  • Received:2012-01-16 Revised:2012-04-06 Published:2012-05-25 Online:2012-05-25

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摘要： 目的观察超短波对脑缺血再灌注后大鼠海马、纹状体和运动皮质内肿瘤坏死因子-α (TNF-α)及Bcl-2 蛋白表达的影响。方法应用线栓法制备大鼠脑缺血再灌注模型，分为假手术组、造模组、超短波治疗组，每组6 只。脑组织切片行免疫组织化学染色(SABC 法)，观察TNF-α和Bcl-2 在脑组织中表达的变化。结果造模组大鼠海马、纹状体和运动皮质TNF-α和Bcl-2 的表达明显高于假手术组(P<0.01)；超短波治疗组TNF-α表达低于造模组，Bcl-2 表达高于造模组(P<0.05)。结论超短波治疗通过影响TNF-α和Bcl-2 的表达对缺血再灌注脑组织产生一定的保护作用。

关键词: 脑缺血, 缺血再灌注, 超短波, 肿瘤坏死因子, Bcl-2

Abstract: Objective To observe the effects of ultrashort wave diathermy on the expression of tumor necrosis factor-α (TNF-α) andBcl-2 in hippocampus, striatum and motor cortex of rats with cerebral ischemia/reperfusion. Methods The model of focal ischemia/reperfusionin Sprague-Dawley rats was induced with intraluminal middle cerebral artery occlusion (MCAO) with nylon monofilament suture. Therats were divided into 3 groups: sham group (n=6), model group (n=6) and ultrashort wave diathermy group (n=6). Brain tissue slices wereimmunohistochemically stained (SABC) to observe the expression of TNF-α and Bcl-2. Results The expression of TNF-α and Bcl-2 in lefthippocampus, striatum and motor cortex was higher in the model group than in the sham group (P<0.01). The expression of TNF-α was lowerbut Bcl-2 was higher in the ultrashort wave diathermy group than in the model group (P<0.05). Conclusion Ultrashort wave diathermycan affect the expression of TNF-α and Bcl-2, which may associate to the neuroprotection from focal cerebral ischemia/reperfusion injury.

Key words: cerebral ischemia, ischemia/reperfusion, ultrashort wave diathermy, tumor necrosis factor, Bcl-2