《中国康复理论与实践》

《中国康复理论与实践》 ›› 2012, Vol. 18 ›› Issue (4): 324-328.

• 论文 • 上一篇    下一篇

中药筋脉通对高糖培养海马神经元细胞Bax、Bcl-2 和Caspase-3蛋白表达的影响

郭蕾蕾1a，张宏1b，田国庆1a，梁晓春1a   

  1. 1.中国医学科学院，北京协和医学院，a：北京协和医院；b：基础医学研究所，北京市 100730。
  • 收稿日期:2012-02-13 修回日期:1900-01-01 出版日期:2012-04-25 发布日期:2012-04-25

Effects of Jinmaitong on Expression of Bax, Bcl-2 and Caspase-3 in Hippocampal Neurons Cultured with High Glucose

GUO Lei-lei, ZHANG Hong, TIAN Guo-qing, et al.   

  1. Peking Union Medical College Hospital and Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China
  • Received:2012-02-13 Revised:1900-01-01 Published:2012-04-25 Online:2012-04-25

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被引次数

19

摘要： 目的探讨中药筋脉通含药血清对高糖培养海马神经元细胞的保护作用。方法采用血清药理学方法制备筋脉通含药血清(JMT)，原代培养新生鼠海马神经元细胞分别接种于不同条件的培养基中，分为正常对照组、高糖组、阳性对照组以及JMT大、中、小剂量组。不同培养基中培养72 h 后使用Western blotting 检测其Bax、Bcl-2 和Caspase-3 蛋白的表达。结果与正常对照组相比，高糖组Caspase-3、Bax 表达明显增加(P<0.01)，Bcl-2 的表达下降(P<0.05)；与高糖组相比，阳性对照组和JMT 各剂量组的Caspase-3、Bax 表达明显下降(P<0.01)，Bcl-2 的表达升高(P<0.05)；与阳性对照组相比，JMT 各剂量组的Caspase-3、Bax 表达下降(P<0.05)，筋脉通大、中剂量组Bcl-2 的表达增加(P<0.05)。结论筋脉通含药血清可能通过提高Bcl-2 蛋白的表达，减少Bax、Caspase-3 的表达，上调Bcl-2/Bax 的比值，从而减少海马神经元细胞的凋亡。

关键词: 糖尿病, 认知功能障碍, 筋脉通, 海马神经元, Bax, Bcl-2, Caspase-3

Abstract: Objective To explore the effect of Jinmaitong (JMT) on expression of Bax, Bcl-2 and Caspase-3 in hippocampal neurons culturedwith high glucose. Methods Hippocampal neurons were primarily cultured and purified from the hippocampus of new-born SpragueDawley rats. Neuron-specific enolase immunocytochemical method was adopted for the identification of the neurons. They were divided intonormal control group, high glucose group, high-dose JMT (JH) group, middle-dose JMT (JM) group, low-dose JMT (JL) group and a positivecontrol group. 72 hours later, Western blotting was adopted to detect the expression of Bax, Bcl-2 and Caspase-3. Results Comparedwith the normal control group, the expression of Bax and Caspase-3 in the high glucose group significantly increased (P<0.01), and the expressionof Bcl-2 significantly decreased (P<0.05). Compared with the high glucose group, the expression of Caspase-3 and Bax in the positivecontrol group and JH, JM, and JL groups significantly decreased (P<0.01), and the expression of Bcl-2 significantly increased (P<0.05).Compared with the positive control group, the expression of Caspase-3 and Bax significantly decreased in JH, JM, and JL groups (P<0.05),and the expression of Bcl-2 significantly increased in JH and JM groups (P<0.05). Conclusion JMT may reduce apoptosis by inhibiting theexpression of Bax and Caspase-3 and promoting the expression of Bcl-2.

Key words: diabetes mellitus, cognitive dysfunction, Jinmaitong, hippocampal neurons, Bax, Bcl-2, Caspase-3